Product Name:
CTNNB1-pY489
Product Number:
ab-pn745
Target Full Name: Catenin (cadherin-associated protein) beta 1
Target Alias: Beta-catenin; Catenin, beta; CATNB; CTNB1; CTNNB
Product Type Specific: CTNNB1 phosphosite-specific antibody
Antibody Code: PN745
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y489
Protein UniProt: P35222
Protein SigNET: CTNNB1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CTNNB1
Antibody Immunogen Sequence: VRLH(pY)GLP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues V485 to P492. T489 phosphorylation induces transcription. This is the major in vivo phosphorylation site in CTNNB1 (≥90 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in sea urchin. CTNNB1 is known to be phosphorylated at this site in vitro by Abl1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Sea urchin.
Related Product 1: CTNNB1 S33+S37 phosphorylation antibody (Cat. No.: AB-PN816)
Related Product 2: CTNNB1 S552 phosphorylation antibody (Cat. No.: AB-PN945)
Related Product 3: CTNNB1 Y654 phosphorylation antibody (Cat. No.: AB-PN579)
Related Product 4: CTNNB1 S675 phosphorylation antibody (Cat. No.: AB-PN946)
Scientific Background: CTNNB1 (Catenin beta-1) is an 88 kDa actin-binding protein and transcription factor that is a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. It features consists of an N-terminal domain, a central "armadillo" (ARM) domain composed of 12 repeats that form a rigid, curved structure for binding partners, and a C-terminal domain. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, allowing activation of Wnt responsive genes, including c-Myc an Cyclin D1. It also acts as a coactivator for other transcription factors, such as NR5A2. It is involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. It also functions as a negative regulator of centrosome cohesion. It is also implicated early embryonic development and tumorigenesis. For example, it acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4. CTNNB1 is phosphorylated and destabilized by CK1 and GSK-3beta. Stabilization of cytoplasmic beta-catenin is a hallmark of a variety of cancers. It blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. It also interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Aberrant activation or mutation of CTNNB1 is strongly associated with various cancers (e.g., colorectal, ovarian, liver). Conversely, reduced levels lead to CTNNB1 syndrome, a neurodevelopmental disorder. This description may include information annotated by UniProt and/or Google AI.