Product Name:
ATASE-pT356
Product Number:
ab-pn742
Target Full Name: Glutamine phosphoribosylpyrophosphate amidotransferase
Target Alias: Amidophosphoribosyltransferase; Glutamine phosphoribosylpyrophosphate amidotransferase; PPAT
Product Type Specific: ATASE (PPAT) phosphosite-specific antibody
Antibody Code: PN742
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T356
Protein UniProt: Q06203
Protein SigNET: ATASE
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ATASE
Antibody Immunogen Sequence: VGR(pT)FIQ(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues V353 to Q359. The effect of T356 phosphorylation is unclear. Phosphorylation of this site in ATASE has been reported in only one mass spectrometry study. This human phosphosite is highly conserved in vertebrates and also found in yeast.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Sea urchin | Budding yeast.
Scientific Background: ATASE (Amidophosphoribosyltransferase; GPAT) is a 57 kDa metabolic enzyme that carries out the first committed, rate-limiting step in de novo purine nucleotide biosynthesis. ATASE catalyzes the reversible formation of 5-phosphoribosyl-1-amine (PRA) from phosphoribosylpyrophosphate (PRPP) and glutamine in the reversible reaction: 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate <=> 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O. PRA is essential for DNA/RNA synthesis and cell proliferation. The active form is often a tetramer (sometimes referred to as a "doughnut-shaped" molecule) consisting of subunits organized into two domains. The protein binds [4Fe-4S] clusters (one per subunit) and requires Mg2+ (or Mn2+) for activity. High levels of PRPP activate ATASE by triggering a structural change that lowers the Km for glutamine by 100-fold. It is inhibited by downstream purine nucleotides, specifically AMP, GMP, and IMP. The cytosolic enzyme is ubiquitously expressed in tissues. This description may include information annotated by UniProt and/or Google AI.