Product Name:
ARID1A-pS363
Product Number:
ab-pn740
Target Full Name: AT-rich interactive domain-containing protein 1A
Target Alias: ARI1A; AT rich interactive domain 1A; B120; BAF250; BAF250a; BRG1-associated factor 250; BRG1-associated factor 250a; C10rf4; C1orf4; HELD; Osa homolog 1; P270; SMARCF1; SMF1; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; SWI-like; SWI-like protein; SWI-SNF complex protein p270
Product Type Specific: ARID1A phosphosite-specific antibody
Antibody Code: PN740
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S363
Protein UniProt: O14497
Protein SigNET: ARID1A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ARID1A
Antibody Immunogen Sequence: HAPM(pS)PGS(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues H359 to S366. The effect of S363 phosphorylation is unclear. This is one of the major in vivo phosphorylation sites in ARID1A (≥89 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is not conserved in mammals, but is found in the mouse. ARID1A is known to be phosphorylated at this site in vitro by CDK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse.
Related Product 1: ARIDA1 Y1506 phosphorylation antibody (Cat. No.: AB-PN864)
Scientific Background: ARID1A (AT-rich interactive domain-containing protein 1A) is a component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. It is a member of the the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex) families. It is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells, and with CREST plays a role regulating the activity of genes essential for dendrite growth. It is highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. This description may include information annotated by UniProt and/or Google AI.