Product Name:
APC-pS2129
Product Number:
ab-pn739
Target Full Name: Adenomatous polyposis coli protein
Target Alias: Adenomatous polyposis coli; Adenomatous polyposis of the colon gene; APC protein; Deleted in polyposis 2.5; DP2; DP2.5; DP3; MAPC
Product Type Specific: APC phosphosite-specific antibody
Antibody Code: PN739
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S2129
Protein UniProt: P25054
Protein SigNET: APC
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human APC
Antibody Immunogen Sequence: RQA(pS)SDS(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R2126 to S2132. The effect of S2129 phosphorylation is unclear. This is one of the major in vivo phosphorylation sites in APC(≥40 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in mammals, and is found in the chicken. APC is known to be phosphorylated at this site in vitro by MAPKAPK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken.
Scientific Background: APC (Adenomatous polyposis coli protein) is a tumour suppressor protein that promotes rapid degradation of CTNNB1 and participates in Wnt signalling as a negative regulator. APC activity is correlated with its phosphorylation by GSK3. It activates the GEF activity of SPATA13 and ARHGEF4, functions in hepatocyte growth factor (HGF)-induced cell migration, and is required for MMP9 up-regulation via the JNK signalling pathway in colorectal tumour cells. It also associates with both microtubules and actin filaments, components of the cytoskeleton, and mediates the organization of F-actin into ordered bundles. APC serves as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. APC mutations often result in truncation of the APC product, and most mutations have occurred within the first half of the coding sequence, and somatic mutations in colorectal tumours are further clustered in a particular region, called MCR (mutation cluster region). The location of germline mutations tends to correlate with the number of colorectal polyps in FAP1 patients. Inactivation of both alleles of the APC gene seems to be required as an early event to develop most adenomas and carcinomas in the colon and rectum as well as some of those in the stomach. This description may include information annotated by UniProt and/or Google AI.