Product Name:
ACO1-pS806
Product Number:
ab-pn737
Target Full Name: Cytoplasmic aconitate hydratase
Target Alias: Aconitase; Aconitate hydratase; Citrate hydro-lyase; EC 4.2.1.3; Ferritin repressor protein; IRE1; IRE-BP 1; IREBP1; Iron regulatory protein 1; IRP1
Product Type Specific: ACO1 phosphosite-specific antibody
Antibody Code: PN737
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S806
Protein UniProt: P21399
Protein SigNET: ACO1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ACO1
Antibody Immunogen Sequence: IHR(pS)NLVGC
Antibody Immunogen Description: Corresponds to amino acid residues I803 to G810. The effect of S806 phosphorylation is unclear. This is phosphorylation site in ACO1 has only been reported in one mass spectrometry study in PhosphoSitePlus. This human phosphosite is highly conserved in vertebrates and found in fish.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish | Fruit fly | Honey bee | Nematode worm | Thale cress.
Related Product 1: ACO1 S711 phosphorylation antibody (Cat. No.: AB-PN736)
Scientific Background: ACO1 (aconitate hydrolase) is a 98-100 kDa cytoplasmic, bifunctional iron sensor that switches between two activities depending on iron availability. When iron is abundant, it binds a 4Fe-4S cluster and acts as a cytoplasmic aconitase. Under iron deprivation, it promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. It binds to iron-responsive elements (IRES) in the 5' or 3' untranslated region of target mRNAs to prevent the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA. It converts citrate to D-threo-isocitrate. Aberrant ACO1 function is linked to iron disorders, anemia, and neurodegenerative conditions, with variants associated with hemoglobin concentrations. This description may include information annotated by UniProt and/or Google AI.