Product Name:
ACC2-pT1342
Product Number:
ab-pn734
Target Full Name: Acetyl CoA carboxylase beta
Target Alias: ACACB; ACCB; ACC-beta; Acetyl-Coenzyme A carboxylase beta; COA2; HACC275
Product Type Specific: ACACB phosphosite-specific antibody
Antibody Code: PN734
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T1342
Protein UniProt: O00763
Protein SigNET: ACC2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ACC2
Antibody Immunogen Sequence: ISI(pT)NPD(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues I1339 to D1345. The effect of T1342 phosphorylation is unclear. This is the major site of phosphorylation in ACC2 by mass spectrometry (≥30 reports). This human phosphosite is highly conserved in mammals and also found in the platypus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.9 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Mouse | Platypus .
Related Product 1: ACC1 S29 phosphorylation antibody (Cat. No.: AB-PN733)
Related Product 2: ACACA S80 phosphorylation antibody (Cat. No.: AB-PN863)
Scientific Background: ACACB (acetyl-CoA carboxylase-2; ACC2) is a mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA ((ATP + acetyl-CoA + HCO3- <=> ADP + phosphatse + malonyl-CoA)) and plays a central role in fatty acid metabolism, the first and rate-limiting step of de novo fatty acid biosynthesis. The two step reaction starts with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. It features a central biotin carboxyl carrier (BCC) domain, an N-terminal biotin carboxylase (BC) domain, and a C-terminal carboxyl transferase (CT) domain. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, it is thought to negatively regulate fatty acid oxidation. Unlike its cytosolic isoform ACACA, ACACB is located on the outer mitochondrial membrane, anchored by a unique N-terminal sequence. Along with its cytosolic isozyme ACACA, it promotes lipid storage. It is inhibited by long-chain fatty acyl CoAs and activated by citrate. It is widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon. Isoform 3 is expressed highly in adipose tissue, and at lower levels in heart, liver, skeletal muscle and testis. This description may include information annotated by UniProt and/or Google AI.