Product Name:
STAG2-pY433
Product Number:
ab-pn729
Target Full Name: Putative uncharacterized protein DKFZp781H1753
Product Type Specific: STAG2 phosphosite-specific antibody
Antibody Code: PN729
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y433
Protein UniProt: Q8N3U4
Protein SigNET: STAG2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STAG2
Antibody Immunogen Sequence: CGEFL(pY)KKL
Antibody Immunogen Description: Corresponds to amino acid residues G429 to L436. The effect of Y433 phosphorylation is unclear. This is the major site of in vivo phosphorylation of STAG2 (≥42 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in the frog.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog.
Scientific Background: STAG2 (Stromal Antigen 2) is a nuclear core subunit of the cohesin complex (along with SMC1, SMC3, and RAD21) and belongs to the SCC3 family. It is essential for sister chromatid cohesion during cell division, after DNA replication, DNA damage repair, and gene expression regulation. The cohesin complex forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. As a crucial component of mitosis and meiosis, it ensures proper chromosome segregation. Mutations in STAG2 are associated with various cancers (e.g., bladder cancer, AML) and developmental disorders. While similar to STAG1, STAG2 has distinct roles in 3D genome organization in the mitotic cohesin complex, such as regulating chromatin loops. Loss or mutation of STAG2 is linked to aneuploidy (abnormal chromosome number) in cancer, and it acts as a tumour suppressor, particularly in bladder cancer. It is also implicated in developmental disorders like Cornelia de Lange syndrome. This description may include information annotated by UniProt and/or Google AI.