Product Name:
SOCS7-pY561
Product Number:
ab-pn728
Target Full Name: Suppressor of cytokine signaling 7
Target Alias: NAP4; NAP-4; Nck, Ash and phospholipase C binding protein; Nck, Ash and phospholipase C gamma-binding protein; NCKAP4; Nck-associated protein 4; SOCS6
Product Type Specific: SOCS7 phosphosite-specific antibody
Antibody Code: PN728
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y561
Protein UniProt: O14512
Protein SigNET: SOCS7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SOCS7
Antibody Immunogen Sequence: EEV(pY)LSL(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E558 to L564. The effect of Y561 phosphorylation is unclear. This is the major site of in vivo phosphorylation of SOCS7 (≥36 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in fish.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Zebra fish | Tiger blowfish.
Scientific Background: SOCS7 (Suppressor of Cytokine Signaling 7; NAP4) is an E3 ubiquitin ligase-substrate adaptor. It regulates signaling pathways—particularly insulin, growth hormone, and prolactin—by mediating the ubiquitination and degradation of target proteins like IRS1 or by sequestering STAT3/5 in the cytoplasm. It features an SH2 domain for binding phosphorylated proteins and a SOCS box for recruiting Elongin B/C ubiquitin ligase machinery. It acts as a scaffold in an ECS(SOCS7) complex, mediating degradation of targets such as phosphorylated DAB1 (crucial for neural development) and IRS1 (insulin signaling). It regulates glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. It Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. It is involved in neuronal migration and fat cell differentiation. It may be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It is highly expressed in the fetal brain during development, specifically the cortical plate. It is also expressed in adult brain and leukocytes. Increased levels are found in Alzheimer's brains. It is also linked to various cancers (breast, pancreas). This description may include information annotated by UniProt and/or Google AI.