Product Name:
SMARCB1-pT134
Product Number:
ab-pn727
Target Full Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
Target Alias: BAF47; INI1; integrase interactor 1; SNF5; SNF5L1
Product Type Specific: SMARCB1 phosphosite-specific antibody
Antibody Code: PN727
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T134
Protein UniProt: Q12824
Protein SigNET: SMARCB1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SMARCB1
Antibody Immunogen Sequence: WVP(pT)LPNSC
Antibody Immunogen Description: Corresponds to amino acid residues W131 to S138. The effect of T134 phosphorylation is unclear. In vivo phosphorylation of SMARCB1 is not commonly detected (≥7 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and found in the frog.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: SMARCA4 S610+S613 phosphorylation antibody (Cat. No.: AB-PN726)
Scientific Background: SMARCB1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1) is a 47-kDa core protein of the SWI/SNF (BAF) chromatin remodeling complex. It belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It acts as a tumour suppressor by regulating gene expression, DNA repair, and cell differentiation. It is essential for normal cell differentiation. Its absence leads to developmental defects and rapid tumour formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. It stimulates in vitro the remodeling activity of SMARCA4. It is involved in activation of CSF1 promoter. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCB1 plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. It is a component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. It is a component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. It also interacts with double-stranded DNA, EphB4, MAEL and GADD34. It acts as a tumour suppressor, in part, by repressing Cyclin D1, thus inhibiting cell cycle progression. Mutations or deletions in SMARCB1 (located on chromosome 22q11.2) are the primary cause of rhabdoid tumours, renal medullary carcinoma, and schwannomatosis. Loss of SMARCB1 is also heavily implicated in aggressive pediatric cancers, such as malignant rhabdoid tumours (MRT) and atypical teratoid rhabdoid tumours (ATRT). This description may include information annotated by UniProt and/or Google AI.