Product Name:
SMAD4-pY513
Product Number:
ab-pn725
Target Full Name: Mothers against decapentaplegic homologue 4
Target Alias: Deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; Deletion target in pancreatic carcinoma 4 homologue; DPC4; Dpc4; MADH4; Madh4; Mothers against decapentaplegic 4; Mothers against decapentaplegic homolog 4; Mothers against DPP 4; SMA4; Sma4; SMAD family member 4
Product Type Specific: Smad4 phosphosite-specific antibody
Antibody Code: PN725
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y513
Protein UniProt: Q13485
Protein SigNET: SMAD4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SMAD4
Antibody Immunogen Sequence: CGPD(pY)PRQ
Antibody Immunogen Description: Corresponds to amino acid residues G510 to Q516. The effect of phosphorylation of Y513 is unclear, and it is a minor site of phosphorylation (≥1 report by mass spectrometry in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus | Frog | Zebra fish | Honey bee.
Related Product 1: Smad3 T8 phosphorylation antibody (Cat. No.: AB-PN658)
Scientific Background: Smad4 (Mothers against decapentaplegic homolog 4; DCP4) is a tumour suppressor protein and transcription factor that belongs to the dwarfin/SMAD family. It functions as the "common-partner" SMAD (co-Smad) in the TGF-β and BMP signaling pathways, mediating cell proliferation, differentiation, and apoptosis by transporting activated receptor-Smads into the nucleus. It forms complexes with receptor-activated Smads (like Smad2/3) to enter the nucleus and bind to DNA. When recruited by MSTN, it promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. It acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. It recognizes for binding SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). It is a component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It is a component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site, and is required for synergistic transcriptional activity in response to TGF-beta. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. It features MH1 and MH2 domains, which when mutated can affect its ability to form complexes or bind DNA. Mutations or deletions of SMAD4 are commonly found in pancreatic, colorectal, and other gastrointestinal cancers. Smad4 acts as a potent tumour suppressor that inhibits cell growth and prevents cancer progression. This description may include information annotated by UniProt and/or Google AI.