Product Name:
PLCE1-pS1096+pT1100
Product Number:
ab-pn721
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1
Target Alias: KIAA1516; Nephrosis type 3; NPHS3; Pancreas-enriched phospholipase C; Phosphoinositide-specific phospholipase C epsilon-1; Phospholipase C, epsilon 1; PLCE; PLC-epsilon-1
Product Type Specific: PLCE1 phosphosite-specific antibody
Antibody Code: PN721
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1096+T1100
Protein UniProt: Q9P212
Protein SigNET: PLCE1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PLCE1
Antibody Immunogen Sequence: RGE(pS)GEV(pT)DDE(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R1093 to E1103. The effect of S1096 and T1100 phosphorylation is unclear. These are two of the major in vivo phosphorylation sites in PLCE1 (≥19 and >5 reports, respectively, by mass spectrometry). These human phosphosites are conserved in mammals and found in the rat.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse.
Related Product 1: PLCB3 S1105 phosphorylation antibody (Cat. No.: AB-PN719)
Related Product 2: PLCG1 Y771+Y775 phosphorylation antibody (Cat. No.: AB-PN774)
Related Product 3: PLCG1 Y783 phosphorylation antibody (Cat. No.: AB-PN530)
Related Product 4: PLCG1 Y977 phosphorylation antibody (Cat. No.: AB-PN722)
Related Product 5: PLCG2 Y1217 phosphorylation antibody (Cat. No.: AB-PN776)
Related Product 6: PLCG2 Y753 phosphorylation antibody (Cat. No.: AB-PN723)
Related Product 7: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN531)
Related Product 8: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN775)
Scientific Background: PLCE1 (Phospholipase C epsilon-1) is a 230 kDa bifunctional enzyme that acts as both a lipase, generating DAG and IP3 to regulate cell signaling, and a Guanine Nucleotide Exchange Factor (GEF) for Ras family proteins. It activates the Ras/MAPK signaling pathway and controls Ca2+ signaling, impacting cell survival and actin organization. It features an N-terminal RasGEF domain, central catalytic X and Y domains, and two C-terminal Ras-associating (RA) domains. Unlike other PLCs, it lacks a pleckstrin homology domain. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. It regulates cell growth, differentiation, and motility, primarily in the heart, kidney, and digestive tract. It is highly expressed in the heart, brain, lung, and testis, with significant roles in cardiomyocyte function and kidney podocyte development. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen. Mutations in the PLCE1 gene are linked to early-onset nephrotic syndrome and glomerular disease (e.g., diffuse mesangial sclerosis). Overexpression or mutations of PLCE1 are associated with various cancers, including gastric, esophageal, and colorectal cancer, as well as skin inflammation. This description may include information annotated by UniProt and/or Google AI.