Product Name:
PLCB3-pS1105
Product Number:
ab-pn719
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3
Target Alias: 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase beta 3; EC 3.1.4.11; Phosphoinositide phospholipase C; Phospholipase C, beta 3 (phosphatidylinositol-specific); Phospholipase C-beta-3; PLC-beta3; PLC-beta-3
Product Type Specific: PLCB3 phosphosite-specific antibody
Antibody Code: PN719
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1105
Protein UniProt: Q01970
Protein SigNET: PLCB3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PLCB3
Antibody Immunogen Sequence: RHN(pS)ISEAC
Antibody Immunogen Description: Corresponds to amino acid residues R1102 to A1109. S1105 phosphorylation inhibits enzyme activity. This is the major in vivo phosphorylation site in PLCB3 (≥232 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is conserved in mammals and also found in the rat. PLCB3 is known to be phosphorylated at this site in vitro by PKACa (PRKACA), PKCa (PRKCA), PKCb1 (PRKCB1), PKCd (PRKCD), PKCe (PRKCE), PKG1 (PRKG1), and PKG2 (PRKG2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Zebra fish.
Related Product 1: PLCE1 S1096+T1100 phosphorylation antibody (Cat. No.: AB-PN721)
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Related Product 3: PLCG1 Y783 phosphorylation antibody (Cat. No.: AB-PN530)
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Related Product 5: PLCG2 Y1217 phosphorylation antibody (Cat. No.: AB-PN776)
Related Product 6: PLCG2 Y753 phosphorylation antibody (Cat. No.: AB-PN723)
Related Product 7: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN531)
Related Product 8: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN775)
Scientific Background: PLCB3 (Phospholipase C-β3) is an essential effector enzyme that mediates G protein-coupled receptor (GPCR) signaling by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2} into secondary messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It is activated by G-alpha-q, G-alpha -11, and G-beta/gamma subunits. It regulates crucial cellular processes like calcium release, protein kinase C (PKC) activation, and trans-Golgi network transport. PLCB3 is a key signaling enzyme that initiates intracellular, receptor-mediated transduction in response to hormones and neurotransmitters. It is particularly critical for mediating inflammatory responses and cellular signaling. PLCB3 plays a significant role in inflammation, particularly in the lungs. It acts on the membrane surface, where its activity is regulated by a "plug" domain that, when removed by membrane binding, allows for interaction with activated G proteins. It features a pleckstrin homology (PH) domain that mediates binding to complexed G-beta/gamma subunits. Its activity is further controlled by calmodulin and it can form homodimers. Mutations or loss of function in PLCB3 are associated with Spondylometaphyseal Dysplasia with Corneal Dystrophy (SMDCD), a disorder characterized by growth deficiencies, skeletal abnormalities, and eye issues. This description may include information annotated by UniProt and/or Google AI.