Product Name:
PFKP-pS386
Product Number:
ab-pn716
Target Full Name: 6-phosphofructokinase type C
Target Alias: 6-phosphofructokinase type C: Phosphofructokinase 1: Phosphohexokinase: Phosphofructo-1-kinase isozyme C: 6-phosphofructokinase, platelet type: Phosphofructokinase-P: Phosphofructokinase, platelet; 6-phosphofructokinase, platelet type; K6PP; PFK, platelet type; PFK-C; PFKF; Phosphofructo-1-kinase isozyme C; Phosphofructokinase 1; Phosphofructokinase, platelet; Phosphofructokinase, platelet type; Phosphohexokinase
Product Type Specific: PFKP phosphosite-specific antibody
Antibody Code: PN716
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S386
Protein UniProt: Q01813
Protein SigNET: PFKP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PFKP
Antibody Immunogen Sequence: RGR(pS)FAG(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R383 to G389. The effect of S386 phosphorylation is unclear. This is the major in vivo phosphorylation site in PFKP (≥234 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in the fruit fly.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Platypus | Chicken | Frog | Zebra fish | Fruit fly.
Related Product 1: PFKFB3 S461 phosphorylation antibody (Cat. No.: AB-PN715)
Related Product 2: PFKFB3 S467 phosphorylation antibody (Cat. No.: AB-PN769)
Related Product 3: PFKL S775 phosphorylation antibody (Cat. No.: AB-PN770)
Related Product 4: PFKP Y651 phosphorylation antibody (Cat. No.: AB-PN771)
Scientific Background: PFKP (ATP-dependent 6-phosphofructokinase, platelet type) is a key regulatory 85 kDa homotetramic enzyme that belongs to the phosphofructokinase type A (PFKA) family, ATP-dependent PFK group I subfamily. It catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. It is highly expressed in platelets, fibroblasts, and various cancer cells, where it promotes proliferation, migration, and survival. While primarily in the cytoplasm, it also operates at the mitochondria, lysosomal membrane, and nucleus. Its activity is enhanced by factors such as c-Myc and AKT, supporting tumour growth. It plays a central role in the Warburg effect (aerobic glycolysis) in cancer, with high expression in breast, lung, and brain tumours. This description may include information annotated by UniProt and/or Google AI.