Product Name:
PFKFB3-pS461
Product Number:
ab-pn715
Target Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Target Alias: 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; 6-phosphofructo-2-kinase; F263; Fructose-2,6-bisphosphatase; IPFK-2; PFK-2/FBPase-2, brain/placenta-type; PFKFB-3
Product Type Specific: PFKFB3 phosphosite-specific antibody
Antibody Code: PN715
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S461
Protein UniProt: Q16875
Protein SigNET: PFKFB3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PFKFB3
Antibody Immunogen Sequence: RRN(pS)VTP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R458 to P464. S461 phosphorylation stimulates enzyme activity. This is the major in vivo phosphorylation site in PFKFB3 (≥125 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is conserved in many vertebrates and found in fish. PFKFB3 is known to be phosphorylated at this site in vitro by Akt1 (PKBa), AMPKa1 (PRKAA1), MAPKAPK2, PKACa (PRKACA), and PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Zebra fish.
Related Product 1: PFKFB3 S467 phosphorylation antibody (Cat. No.: AB-PN769)
Related Product 2: PFKL S775 phosphorylation antibody (Cat. No.: AB-PN770)
Related Product 3: PFKP S386 phosphorylation antibody (Cat. No.: AB-PN716)
Related Product 4: PFKP Y651 phosphorylation antibody (Cat. No.: AB-PN771)
Scientific Background: PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3) is a bifunctional enzyme that belongs to the phosphoglycerate mutase family. It is a key metabolic regulator that synthesizes fructose-2,6-bisphosphate (F2,6BP), a potent activator of glycolysis. It serves as a, "master regulator" of glycolysis. It has high kinase activity to produce F2,6BP, which activates 6-phosphofructo-1-kinase (PFK-1), the rate-limiting enzyme of glycolysis. Beyond glycolysis, PFKFB3 can localize to the nucleus, where it activates Cyclin-dependent kinase 1 (Cdk1) and regulates cell cycle progression. It exists in several isoforms, with the carboxyl-terminal domain influencing its localization and activity. It is highly expressed in solid tumours, leukemia, and rapidly proliferating cells. It promotes high glycolytic flux, cell proliferation, and survival. It sustains the high glycolytic rate characteristic of the Warburg effect in cancer cells, aiding tumour growth and angiogenesis. Its inhibition leads to apoptosis and cell cycle arrest. This description may include information annotated by UniProt and/or Google AI.