Product Name:
NOS2-pS745
Product Number:
ab-pn711
Target Full Name: Nitric oxide synthase, inducible
Target Alias: EC 1.14.13.39; Hepatocyte NOS; HEP-NOS; Inducible; Inducible NOS; Nitric oxide synthase 2, inducible; NOS; NOS type II; NOS2A
Product Type Specific: NOS2 phosphosite-specific antibody
Antibody Code: PN711
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S745
Protein UniProt: P35228
Protein SigNET: NOS2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NOS2
Antibody Immunogen Sequence: NLQ(pS)PTS(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues N742 to S748. S745 phosphorylation stimulates enzyme activity. This phosphorylation site appears to be poorly detected by mass spectrometry (≥1 report ). This human phosphosite is highly conserved in vertebrates and found in the frog. NOS2 is known to be phosphorylated at this site in vitro by ERK1 (MAPK3), and ERK2 (MAPK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: NOS3 T1175+S1177 phosphorylation antibody (Cat. No.: AB-PN712)
Related Product 2: NOS1 S746 phosphorylation antibody (Cat. No.: AB-PN762)
Related Product 3: NOS2 Y151 phosphorylation antibody (Cat. No.: AB-PN763)
Scientific Background: NOS2 (Nitric oxide synthase 2; iNOS) is an inducible, calcium-independent enzyme that generates large amounts of nitric oxide (NO) for immune defense and inflammatory responses. NO mediates tumouricidal and bactericidal actions. Unlike constitutive isoforms (NOS1, NOS3), NOS2 expression is induced by inflammatory stimuli, such as cytokines (TNF-alpha, IL-1-beta) and bacterial lipopolysaccharide (LPS). As a 1153-amino acid, heme-containing homodimer, it catalyzes the conversion of L-arginine to L-citrulline and NO. In macrophages, it functions as an oxidoreductase, using cofactors like NADPH, FAD, FMN, and tetrahydrobiopterin (BH4) to produce nitric oxide. The catalyzed reaction is: 2 L-arginine + 3 NADPH + 4 O2 + H+ = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. It also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. It features a C-terminal reductase domain and an N-terminal oxygenase domain. It is a component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity, and probably multiple targets including ANXA5, EZR, MSN and VIM. It is involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8. It is expressed in the liver, retina, bone cells and airway epithelial cells of the lung, but not in platelets. While protective against pathogens (viruses, bacteria, fungi), excessive NOS2-derived NO is associated with inflammatory diseases, sepsis, and cancer. This description may include information annotated by UniProt and/or Google AI.