Product Name:
MSH6-pS14
Product Number:
ab-pn708
Target Full Name: DNA mismatch repair protein Msh6
Target Alias: DNA mismatch repair MSH6; G/T mismatch binding protein; GTBP; GTMBP; MutS 6; MutS homolog 6 (E. coli); MutS-alpha 160 kDa; MutS-alpha 160 kDa subunit; P160
Product Type Specific: MSH6 phosphosite-specific antibody
Antibody Code: PN708
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S14
Protein UniProt: P52701
Protein SigNET: MSH6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MSH6
Antibody Immunogen Sequence: FPK(pS)PAL(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F11 to L17. The effect of S14 phosphorylation is unclear. This is the major in vivo phosphorylation site in MSH6 (≥123 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in fish.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse | Frog | Zebra fish.
Scientific Background: MSH6 (DNA mismatch repair protein Msh6) belongs to the DNA mismatch repair MutS family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MSH2 to form MutS alpha, which detects and repairs single base mismatches and small insertion/deletion loops during DNA replication. It also has roles in DNA damage response, including non-homologous end-joining (NHEJ) of double-strand breaks and promoting apoptosis in response to damage. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and it recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, it forms a ternary complex with the MutL alpha heterodimer, which is believed to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is important for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. Although it is primarily nuclear in location, it is also found in the cytoplasm. MSH6 is strongly expressed in the nuclei of normal proliferating cells, such as those in the colonic epithelium and lymphocytes. Germline mutations in the MSH6 gene are associated with Lynch syndrome, which significantly increases the risk of endometrial, colorectal, and other cancers. This description may include information annotated by UniProt and/or Google AI.