Product Name:
HMGCR-pS872
Product Number:
ab-pn705
Target Full Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
Product Type Specific: HMGCR phosphosite-specific antibody
Antibody Code: PN705
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S872
Protein UniProt: P04035
Protein SigNET: HMGCR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HMGCR
Antibody Immunogen Sequence: HNR(pS)KIN(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues H869 to N875. The effect of S872 phosphorylation is unclear. This phosphorylation site is not commonly detected by mass spectrometry (≥9 reports), but appears to be the major phosphosite on HMG-CoA reductase. This phosphosite is not well conserved in vertebrates, but is found in rhesus monkey. HMGCR is known to be phosphorylated at this site in vitro by AMPKa1 (PRKAA1), PKACa (PRKACA), and PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.9 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly.
Scientific Background: HMGCR (3-Hydroxy-3-methylglutaryl-coenzyme A reductase) is a crucial transmembrane glycoprotein located in the endoplasmic reticulum that acts as the rate-limiting enzyme in cholesterol biosynthesis by converting HMG-CoA to mevalonate, which is also necessary for producing nonsterol isoprenoids as well (e.g., ubiquinone) and farnesylated proteins (e.g. Ras). It catalyzes the reaction: (R)-mevalonate + 2 NADP+ + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+. It is often found as a homodimer or homotetramer. It contains an N-terminal sterol-sensing domain (SSD) and a C-terminal catalytic domain. Its activity is regulated by sterol-dependent degradation, mediated by interactions with INSIG1 (stimulated by cholesterol). It is also inhibited by phosphorylation via AMP-activated protein kinase. It is the primary target of statin drugs, a class of cholesterol-lowering drugs, for treating dyslipidemia and cardiovascular disease. HMGCR mutations cause a form of limb-girdle muscular dystrophy (LGMD R28), and it is the target of autoimmune anti-HMGCR myopathy. Overexpression of HMGCR is also observed in various tumours, promoting cancer cell proliferation and survival. This description may include information annotated by UniProt and/or Google AI.