Product Name:
DCK-pS74
Product Number:
ab-pn696
Target Full Name: Deoxycytidine kinase
Target Alias: EC 2.7.1.74
Product Type Specific: DCK phosphosite-specific antibody
Antibody Code: PN696
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S74
Protein UniProt: P27707
Protein SigNET: DCK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DCK
Antibody Immunogen Sequence: LTM(pS)QKNGGC
Antibody Immunogen Description: Corresponds to amino acid residues L71 to G784. S74 phosphorylation stimulates enzyme activity. This is the major in vivo phosphorylation sites in DCK (≥115 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and found in fish. DCK is known to be phosphorylated at this site in vitro by ATR, and CK1D.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Scientific Background: DCK (Deoxycytidine kinase) is a metabolite kinase that belongs to the the DCK/DGK family. It is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). It has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It catalyzes the reaction: 2'-deoxycytidine + a ribonucleoside 5'-triphosphate = dCMP + a ribonucleoside 5'-diphosphate + H+. It exists as a homodimer, with each subunit containing a nucleotide donor binding site, a nucleoside acceptor binding site, and a base-sensing loop. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. It is primarily located in the nucleus, highly expressed in rapidly dividing cells, including lymphoid cells, and its expression varies across different cancer tissues. DCK is responsible for activating several nucleoside analog drugs (e.g., Gemcitabine, Ara-C). Its deficiency or reduced activity is a key mediator of resistance to these therapies. This description may include information annotated by UniProt and/or Google AI.