Product Name:
COX2-pY446
Product Number:
ab-pn695
Target Full Name: Prostaglandin G/H synthase 2
Target Alias: COX2; Cyclooxygenase-2; PGH synthase 2; PGH2; PGHS-2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2
Product Type Specific: COX2 phosphosite-specific antibody
Antibody Code: PN695
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y446
Protein UniProt: P35354
Protein SigNET: COX2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human COX2
Antibody Immunogen Sequence: (ac)QMK(pY)QSF(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q443 to F449. Y446 phosphorylation activates COX2 enzymatic activity. However, this phosphosite has been poorly detected in mass spectrometry studies (≥1 report by mass spectrometry). The phosphosite is commonly conserved in vertebrates and found in fish. COX2 is known to be phosphorylated at this site in vitro by Fyn.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: COX1 (PTGS1) expression antibody (Cat. No.: AB-NN381-1)
Related Product 2: COX4 (COX4I1) expression antibody (Cat. No.: AB-NN379-1)
Scientific Background: COX2 (Prostaglandin G/H synthase 2; PTGS2) is an inducible enzyme that belongs to the prostaglandin G/H synthase family. It catalyzes the conversion of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)) to prostanoids, a class of C20 oxylipins, including prostaglandin H2 (PGH2). The cyclooxygenase activity of COX2 oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and its peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumour angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, COX2 plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia. PTGS1 and PTGS2 are the targets of nonsteroidal anti-inflammatory drugs (NSAIDs) including aspirin and ibuprofen. Aspirin is able to produce an irreversible inactivation of the enzyme through a serine acetylation. Inhibition of the PGHSs with NSAIDs acutely reduces inflammation, pain, and fever, and long-term use of these drugs reduces the risk of fatal thrombotic events, as well as the development of colon cancer and Alzheimer's disease. This description may include information annotated by UniProt and/or Google AI.