Product Name:
ACP1-pY132+pY133
Product Number:
ab-pn687
Target Full Name: Low molecular weight phosphotyrosine protein phosphatase
Target Alias: Acid phosphatase 1; Acid phosphatase 1, soluble; Adipocyte acid phosphatase; Lmwptp; LMW-PTP; LMW-PTPase; Low molecular weight cytosolic acid phosphatase; PPAC; PTPase; Red cell acid phosphatase 1
Product Type Specific: ACP1 phosphosite-specific antibody
Antibody Code: PN687
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y132+Y133
Protein UniProt: P24666
Protein SigNET: ACP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ACP1
Antibody Immunogen Sequence: EDP(pY)(pY)GND(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E129 to D136. Y132 and Y133 phosphorylation stimulates phosphatase activity, and regulates cell adhesion, cell motility, and transcription. These are two major in vivo phosphorylation site in ACP1 (≥207 and >86 reports, respectively, by mass spectrometry in PhosphoSitePlus). These phosphosites are highly conserved and also found in yeast. ACP1 is known to be phosphorylated in vitro at Y132 by Lck, Src. Y133: Lck, and Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Budding yeast.
Scientific Background: ACP1 (low molecular weight phosphotyrosine protein phosphatase; Acid phosphatase 1) is a widely expressed, 18 kDa enzyme that hydrolyzing protein tyrosine phosphate to protein tyrosine. In addition to dephosphorylating tyrosine phosphorylated proteins, it also targets low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2. It modulates key signaling molecules, including insulin and platelet-derived growth factor (PDGF) receptors. T-lymphocytes express only isoform 2. A third isoform does not possess phosphatase catalytic activity. It is primarily found in the cytosol and nucleoplasm. Deletion of the ACP1 gene has shown resistance to pressure overload hypertrophy and heart failure. It is Involved in osteoporosis and linked to metabolic parameters in Type 1 diabetes. Elevated expression of ACP1 is linked to poor prognosis in prostate and colon cancers. This description may include information annotated by UniProt and/or Google AI.