Product Name:
ACLY-pY682
Product Number:
ab-pn686
Target Full Name: ATP-citrate synthase
Target Alias: ACL; ATP citrate lyase; ATP-citrate (pro-S-)-lyase; ATP-citrate lyase; ATPCL; Citrate cleavage enzyme; CLATP; EC 2.3.3.8
Product Type Specific: ACLY phosphosite-specific antibody
Antibody Code: PN686
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y682
Protein UniProt: P53396
Protein SigNET: ACLY
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ACLY
Antibody Immunogen Sequence: DGV(pY)EGVA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D679 to A686. Phosphorylation of Y682 increases enzyme activity. This is the most frequently phosphorylated site in ATP-citrate lysate in mass spectrometry studies (≥383 reports in PhsophositePlus). This human phosphosite is highly conserved in vertebrates and also found in the nematode worm. ACLY is known to be phosphorylated at this site in vitro by Lyn, Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Nematode worm.
Related Product 1: ACLY S455 phosphorylation antibody (Cat. No.: AB-PN735)
Scientific Background: ACLY (ATP-citrate synthetase) catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA with the reversible reaction: oxaloacetate + acetyl-CoA + ADP + phosphate <=> citrate + ATP + CoA. It is the primary enzyme responsible for synthesizing cytosolic acetyl-CoA, which is essential for de novo lipogenesis and cholesterogenesis. Acetyl-CoA is a common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism. As a homotetramer, it links glucose metabolism to lipid biosynthesis and histone acetylation. It features an N-terminal citrate-binding domain (Rossmann fold), a CoA binding domain, and a C-terminal citrate synthase domain. Found in the cytosol and nucleus of cells, it is highly active in the liver and adipose tissue during carbohydrate feeding. ACLY is upregulated in numerous tumour types, and its inhibition halts cancer cell proliferation and induces apoptosis. This description may include information annotated by UniProt and/or Google AI.