Product Name:
YLPM1-pS561
Product Number:
ab-pn684
Target Full Name: YLP motif-containing protein 1
Target Alias: C14orf170; Nuclear protein ZAP3; YLP motif containing 1; ZAP; ZAP113
Product Type Specific: YLPM1 phosphosite-specific antibody
Antibody Code: PN684
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S561
Protein UniProt: P49750
Protein SigNET: YLPM1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human YLPM1
Antibody Immunogen Sequence: SYLE(pS)PRGC
Antibody Immunogen Description: Corresponds to amino acid residues S557 to G564. The effect of S561 phosphorylation is unclear. This has not been reported as an in vivo phosphorylation site based on high throughput mass spectrometry reports recorded in PhosphoSitePlus. YLPM1 is known to be phosphorylated at this site in vitro by ERK1 (MAPK3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog.
Scientific Background: YLPM1 (YLP motif-containing protein 1; ZAP3) is a 241 kDa nuclear protein that functions in RNA binding and transcriptional regulation, and downregulates telomerase activity (TERT) during stem cell differentiation. It is involved in nuclear speckle organization, acts as a putative nucleoside kinase, interacts with Protein Phosphatase 1 (PP1), and is essential for embryonic development, with knockouts in mice causing early lethality. It plays a role in the reduction of telomerase activity during differentiation of embryonic stem cells by binding to the core promoter of TERT and controlling its down-regulation. It features 4 N-terminal YLP motif repeats, several arginine-glycine-glycine (RG/G) boxes, and a highly conserved C-terminal RVRW motif. It is widely expressed in various human tissues, including the heart, skin, liver, testis, and kidney, and in neuroblastoma and embryonic kidney cell lines. It is associated with Neuronal Ceroid Lipofuscinosis 6A and is crucial for proper embryogenesis. This description may include information annotated by UniProt and/or Google AI.