Product Name:
YAP1-pT119
Product Number:
ab-pn682
Target Full Name: Yorkie homologue
Target Alias: YAP2; YAP65; Yes-associated protein 1, 65kDa
Product Type Specific: YAP1 phosphosite-specific antibody
Antibody Code: PN682
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T119
Protein UniProt: P46937
Protein SigNET: YAP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human YAP1
Antibody Immunogen Sequence: CAGAL(pT)PQHV
Antibody Immunogen Description: Corresponds to amino acid residues A115 to V123. T119 phosphorylation induces cell motility. This is one of the major sites of in vivo phosphorylation of YAP based on ≥135 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus. YAP1 is known to be phosphorylated at this site in vitro by CDK1, ERK1 (MAPK3), JNK1 (MAPK8), and JNK2 (MAPK9).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Chicken.
Related Product 1: YAP1 S109 phosphorylation antibody (Cat. No.: AB-PN681)
Related Product 2: YAP1 S127 phosphorylation antibody (Cat. No.: AB-PN683)
Scientific Background: YAP1 (Yes-associated protein 1) is a 65 kDa transcriptional co-activator and co-repressor that belongs to the YAP1 family, and the primary effector of the Hippo signaling pathway. The Hippo signaling pathway that plays a pivotal role in the control of organ size, as a potent promoter of cell proliferation and tissue regeneration. It contributes to tumour suppression by restriction of cell proliferation and promoting apoptosis. It shuttles between the cytoplasm and nucleus to activate gene expression, particularly through TEAD transcription factors. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1. The activated kinases LATS1/2 phosphorylate YAP1, causing its cytoplasmic retention and degradation. When the pathway is off, YAP1 enters the nucleus, binding to TEADs or other transcription factors (e.g., Runx1/2, p73), which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. It plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. It acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. It functions to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. YAP1 features a WW domain that binds to proline-rich PY motifs (PPxY) on other proteins, and it is also able to interact with the SH3 domain of c-Yes (and also c-Src), through a stretch of proline residues; this proline-rich region is able to interact with SH3 domains of many other proteins. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4. As an oncogene, YAP1 is frequently overactivated in many human cancers, including liver and prostate cancers, leading to tumour growth, metastasis, and therapy resistance. Isoforms lacking the transactivation domain are found in striatal neurons of patients with Huntington disease. This description may include information annotated by UniProt and/or Google AI.