Product Name:
UBF-pT201
Product Number:
ab-pn680
Target Full Name: Nucleolar transcription factor 1
Target Alias: Autoantigen NOR-90; NOR-90; TCFUBF; UBTF; UBF1; UBF-1; Upstream binding factor 1; Upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1
Product Type Specific: UBF phosphosite-specific antibody
Antibody Code: PN680
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T201
Protein UniProt: P17480
Protein SigNET: UBF
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human UBF
Antibody Immunogen Sequence: EKPK(pT)PQQ(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E197 to Q204. T201 phosphorylation inhibits transcriptional activity by inhibiting DNA binding. This is one of the major sites of in vivo phosphorylation of UBF based on ≥12 mass spectrometry reports recorded in PhosphoSitePlus. UBF is known to be phosphorylated at this site in vitro by ERK1 (MAPK3), and ERK2 (MAPK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse | Zebra fish.
Related Product 1: UBF S484 phosphorylation antibody (Cat. No.: AB-PN679)
Scientific Background: Upstream Binding Factor (UBF) is a critical nucleolar phosphoprotein and transcription factor that activates RNA polymerase I by binding to ribosomal DNA (rDNA) promoters and enhancers. It is essential for the expression of 18S, 5.8S, and 28S ribosomal RNAs (rRNA), playing a vital role in ribosome biogenesis. This is mediated through cooperative interactions with the transcription factor SL1/TIF-IB complex for binding to the upstream control element. It acts as an architectural, sequence-tolerant DNA-binding protein containing HMG boxes (high-mobility group boxes) , allowing it to bend, loop, and wrap DNA, creating a structure called an "enhancesome" that resembles a nucleosome, to facilitate the formation of transcriptionally active chromatin. UBF is also facilitates a C-terminal acidic region that helps regulate its localization to the nucleolus. UBF binds to DNA in a sequence-tolerant manner, often interacting with the minor groove. It is considered a primary response gene, with its expression levels correlating with rates of rDNA transcription. There are two main forms in humans (94 and 97 kDa), and in some species, UBF1 is essential for chromatin remodeling, while UBF2 has a different, less active role. UBF also counteracts histone H1-mediated repression of the rDNA promoter. This description may include information annotated by UniProt and/or Google AI.