Product Name:
TP53BP1-pT1056
Product Number:
ab-pn678
Target Full Name: Tumor suppressor p53-binding protein 1
Target Alias: 53BP1; P202; P531; P53-binding protein 1; TP53B; TRP53BP1; Tumor protein p53 binding protein 1
Product Type Specific: TP53BP1 phosphosite-specific antibody
Antibody Code: PN678
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T1056
Protein UniProt: Q12888
Protein SigNET: TP53BP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TP53BP1
Antibody Immunogen Sequence: DPPT(pT)PIRGC
Antibody Immunogen Description: Corresponds to amino acid residues D1052 to G1060. The effect of T1056 phosphorylation is unclear. This is a major site of in vivo phosphorylation of TP53BP1 (≥33reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). TP53BP1 is known to be phosphorylated at this site in vitro by ERK1 (MAPK3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog.
Scientific Background: TP53BP1 (TP53-binding protein 1) is a double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. This ~27 kDa checkpoint protein plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation of TP53BP1 by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. It is recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at Lys-15 (H2AK15Ub) and histone H4 dimethylated at Lys-20 (H4K20me2), which are two histone marks that are present at DSBs sites. It plays a significant role in promoting cell-cycle checkpoints, particularly in response to ionizing radiation (IR), and is essential for maintaining genome stability. It is required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. It participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. It promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1. It contains tandem Tudor domains (critical for recruitment to damage sites) and C-terminal BRCT domains that facilitate interactions with other repair proteins. It is mainly nuclear, but its localization shifts to the cytoplasm during blastocyst formation, and it becomes undetectable at mitotic chromosomes. In the context of cancer, where its deficiency or abnormal expression can lead to genomic instability. This description may include information annotated by UniProt and/or Google AI.