Product Name:
TORC2-pS433
Product Number:
ab-pn677
Target Full Name: CREB regulated transcription coactivator 2
Target Alias: Transducer of regulated cAMP response element-binding protein 2
Product Type Specific: CRTC2 phosphosite-specific antibody
Antibody Code: PN677
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S433
Protein UniProt: Q53ET0
Protein SigNET: TORC2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TORC2
Antibody Immunogen Sequence: RVPL(pS)PLS(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R429 to S436. The effect of S433 phosphorylation is unclear. This is the major site of in vivo phosphorylation of TORC2 (≥114 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). TORC2 is known to be phosphorylated at this site in vitro by CDK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
Scientific Background: TORC2 (CREB-regulated transcription coactivator 2; CRTC2) is a transcriptional coactivator for CREB1 that belongs to the TORC family. CREB1 stimulates transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 cts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' activating phosphorylation. It enhances the interaction of CREB1 with TAF4. It regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway, and the expression of specific genes such as the steroidogenic gene, StAR. It is a potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells, and a coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). It is most abundantly expressed in the thymus, and present in both B and T-lymphocytes. High levels of TORC2 are also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels of TORC2 are found in brain, colon, heart, kidney, prostate, small intestine and stomach, with weak expression in liver and pancreas. This description may include information annotated by UniProt and/or Google AI.