Product Name:
THRAP3-pS253
Product Number:
ab-pn676
Target Full Name: Thyroid hormone receptor-associated protein 3
Target Alias: T150; Thyroid hormone receptor associated protein 3; Thyroid hormone receptor associated protein 3 (TRAP150); Thyroid hormone receptor-associated protein complex 150 kDa component; TR150; TRAP150
Product Type Specific: THRAP3 phosphosite-specific antibody
Antibody Code: PN676
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S253
Protein UniProt: Q9Y2W1
Protein SigNET: THRAP3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human THRAP3
Antibody Immunogen Sequence: PALK(pS)PLQ(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues P249 to Q256. The effect of S253 phosphorylation is unclear. This is one of the major sites of in vivo phosphorylation of THRAP3 (≥148 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). THRAP3 is known to be phosphorylated at this site in vitro by ERK1 (MAPK3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog.
Scientific Background: THRAP3 (Thyroid Hormone Receptor Associated Protein 3; TRAP150) is a ubiquitous 150-kDa nuclear protein that belongs to the BCLAF1/THRAP3 family. It serves as a key transcriptional coactivator and regulator of pre-mRNA splicing, particularly within nuclear speckles, where it is important for maintaining the structure of the nucleolus. It plays essential roles in the DNA damage response, R-loop resolution, and adipocyte differentiation. As a transcriptional coactivation, it interacts with the thyroid hormone receptor (TR) and is involved in mediating gene expression. It functions in RNA processing as a component of the spliceosome, to regulate alternative splicing and mRNA stability. It remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). It can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. It collaborates with BCLAF1 to promote R-loop resolution and maintain genomic stability, with depletion causing hypersensitivity to DNA-damaging agents. It interacts with PPARγ to control diabetic gene programming in adipocytes, and it may play a role in the terminal stage of adipocyte differentiation. During splicing of PTPRC/CD45, THRAP3 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. It is involved in cyclin-D1/CCND1 mRNA stability, probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Cooperatively with HELZ2, it enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. It plays a role in the positive regulation of the circadian clock, by acting as a coactivator of the CLOCK-ARNTL/BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes. It features arginine-serine (RS) rich domains and a LISH domain. THRAP3 is highly expressed in various tissues and is involved in controlling diverse cellular processes, from metabolic regulation to cell survival after stress. This description may include information annotated by UniProt and/or Google AI.