Product Name:
TERF1-pT371
Product Number:
ab-pn675
Target Full Name: Telomeric repeat-binding factor 1
Target Alias: NIMA-interacting protein 2; PIN2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor (NIMA-interacting) 1; Telomeric repeat binding factor 1; TRBF1; TRF; TTAGGG repeat binding factor 1; TTAGGG repeat-binding factor 1
Product Type Specific: TERF1 phosphosite-specific antibody
Antibody Code: PN675
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T371
Protein UniProt: P54274
Protein SigNET: TERF1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TERF1
Antibody Immunogen Sequence: SQPV(pT)PEKH(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues S367 to H375. T371 phosphoryation generates a docking site for Plk1 at S435. This is one of the major sites of in vivo phosphorylation of TERF1. This is a major site of in vivo phosphorylation of TERF1 based on ≥9 mass spectrometry reports recorded in PhosphoSitePlus. TERF1 is known to be phosphorylated at this site in vitro by CDK1 (CDC2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse.
Scientific Background: TERF1 (Telomeric Repeat-Binding Factor 1; TRF1) is a homodimeric component of the shelterin complex (telosome) that binds to double-stranded 5'-TTAGGG-3' telomeric repeats. As part of the shelterin complex, it stabilizes telomeres and regulates access of telomerase. It acts as a negative regulator of telomerase, controlling telomere length by inhibiting elongation and preventing end-to-end fusions. TERF1 protects chromosome ends from degradation and is essential for proper telomere replication and mitotic spindle assembly. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. It is present at telomeres throughout the entire cell cycle. It features a C-terminal Myb DNA-binding motif and an N-terminal dimerization domain (TRFH domain). It is highly and ubiquitously expressed, with the Pin2 isoform most commonly produced. Deletion in mice leads to early embryonic lethality, highlighting its essential role in development. This description may include information annotated by UniProt and/or Google AI.