Product Name:
STAT6-pY641
Product Number:
ab-pn673
Target Full Name: Signal transducer and activator of transcription 6
Target Alias: IL-4 Stat
Product Type Specific: STAT6 phosphosite-specific antibody
Antibody Code: PN673
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y641
Protein UniProt: P42226
Protein SigNET: STAT6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STAT6
Antibody Immunogen Sequence: CGRG(pY)VPAT
Antibody Immunogen Description: Corresponds to amino acid residues G638 to T645. Y641 phosphorylation stimulates transcription and induces DNA and STAT6. This is one of the major sites of in vivo phosphorylation of STAT6 based on ≥96 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse | Chicken.
Related Product 1: STAT1 Y701 phosphorylation antibody (Cat. No.: AB-PN666)
Related Product 2: STAT1 S727 phosphorylation antibody (Cat. No.: AB-PN667)
Related Product 3: STAT2 Y690 phosphorylation antibody (Cat. No.: AB-PN668)
Related Product 4: STAT3 Y705+T708 phosphorylation antibody (Cat. No.: AB-PN539)
Related Product 5: STAT3 S727 phosphorylation antibody (Cat. No.: AB-PN669)
Related Product 6: STAT4 Y693 phosphorylation antibody (Cat. No.: AB-PN670)
Related Product 7: STAT5A Y694 phosphorylation antibody (Cat. No.: AB-PN671)
Related Product 8: STAT5A S780 phosphorylation antibody (Cat. No.: AB-PN672)
Scientific Background: STAT6 (Signal transducer and activator of transcription 6) is a STAT family transcription factor that primarily mediates interleukin 3/ IL-3 signaling and interleukin-4 (IL-4) and plays a critical role in differentiation of T helper 2 (Th2) cells, Th2 immune responses, allergic inflammation, and B-cell immunoglobulin E (IgE) isotype switching. Like other STAT transcription factors, it features an SH2 domain for receptor association and a DNA-binding domain for transcription. Upon tyrosine phosphorylation by JAK's after their activation by IL3 and IL4 receptors, it dimerizes, translocates to the nucleus, and activates target gene expression. It associated with asthma and allergic diseases. In oncology, NAB2-STAT6 fusion proteins are highly specific, driving the development of solitary fibrous tumours (SFTs). This description may include information annotated by UniProt and/or Google AI.