Product Name:
STAT5A-pS780
Product Number:
ab-pn672
Target Full Name: Signal transducer and activator of transcription 5A
Target Alias: Mammary gland factor; MGF; MPF; STA5A; STAT5
Product Type Specific: STAT5A phosphosite-specific antibody
Antibody Code: PN672
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S780
Protein UniProt: P42229
Protein SigNET: STAT5A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STAT5A
Antibody Immunogen Sequence: DSRL(pS)PPAGC
Antibody Immunogen Description: Corresponds to amino acid residues D776 to G784. S780 phosphorylation regulates molecular association, including inhibiting interaction with ERK1. This is a minor site of in vivo phosphorylation of STAT5A (≥25 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). STAT5A is known to be phosphorylated at this site in vitro by CDK8, ERK1 (MAPK3), ERK2 (MAPK1), and PAK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Related Product 1: STAT1 Y701 phosphorylation antibody (Cat. No.: AB-PN666)
Related Product 2: STAT1 S727 phosphorylation antibody (Cat. No.: AB-PN667)
Related Product 3: STAT2 Y690 phosphorylation antibody (Cat. No.: AB-PN668)
Related Product 4: STAT3 Y705+T708 phosphorylation antibody (Cat. No.: AB-PN539)
Related Product 5: STAT3 S727 phosphorylation antibody (Cat. No.: AB-PN669)
Related Product 6: STAT4 Y693 phosphorylation antibody (Cat. No.: AB-PN670)
Related Product 7: STAT5A Y694 phosphorylation antibody (Cat. No.: AB-PN671)
Related Product 8: STAT6 Y641 phosphorylation antibody (Cat. No.: AB-PN673)
Scientific Background: STAT5A (Signal transducer and activator of transcription 5A) is a STAT family transcription factor.that acts as a signaling molecule and transcription activator, crucial for mediating responses to and growth factors by regulating gene expression, primarily promoting cell survival and differentiation. It mediates cellular responses to various cytokines (e.g., IL-2, IL-3, Prolactin) that bind to cytokine receptors, and to growth factors (KITLG/SCF, FGFR1, FGFR2, FGFR3 and FGFR4) that activate receptor-tyrosine kinases (ERBB4). Upon cytokine stimulation, STAT5A is phosphorylated by JAK's on Tyr- 694, forming homo- or heterodimers (typically with STAT5B) that translocate to the nucleus. STAT5A is vital for immune cell function, hematopoietic development, mammary gland development, and lactation. It regulates genes involved in anti-apoptosis (e.g., BCL2L1). It binds to the GAS element and activates PRL-induced transcription. It regulates the expression of milk proteins during lactation. STAT5A shares 96% similarity with its paralog, STAT5B, and both proteins exhibit overlapping functions. STAT5A is implicated in hematological malignancies (leukemia/lymphoma) and, alongside STAT5B, is a target in prostate cancer due to its role in cell proliferation. This description may include information annotated by UniProt and/or Google AI.