Product Name:
STAT4-pY693
Product Number:
ab-pn670
Target Full Name: Signal transducer and activator of transcription 4
Product Type Specific: STAT4 phosphosite-specific antibody
Antibody Code: PN670
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y693
Protein UniProt: Q14765
Protein SigNET: STAT4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STAT4
Antibody Immunogen Sequence: CGDKG(pY)VPSV
Antibody Immunogen Description: Corresponds to amino acid residues G689 to V697. Y693 phosphorylation induces interaction with LIFR, STAT3 and STAT4. This is the major sites of in vivo phosphorylation of STAT4 based on ≥8 mass spectrometry recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Related Product 1: STAT1 Y701 phosphorylation antibody (Cat. No.: AB-PN666)
Related Product 2: STAT1 S727 phosphorylation antibody (Cat. No.: AB-PN667)
Related Product 3: STAT2 Y690 phosphorylation antibody (Cat. No.: AB-PN668)
Related Product 4: STAT3 Y705+T708 phosphorylation antibody (Cat. No.: AB-PN539)
Related Product 5: STAT3 S727 phosphorylation antibody (Cat. No.: AB-PN669)
Related Product 6: STAT5A Y694 phosphorylation antibody (Cat. No.: AB-PN671)
Related Product 7: STAT5A S780 phosphorylation antibody (Cat. No.: AB-PN672)
Related Product 8: STAT6 Y641 phosphorylation antibody (Cat. No.: AB-PN673)
Scientific Background: STAT4 (Signal transducer and activator of transcription 4) is an 86 kDa, STAT family transcription factor that regulates gene expression in response to cytokines, crucial for immune cell maturation and function. It is primarily activated by IL-12, IL-23, and Type I IFNs, promoting T-helper 1 (Th1) cell differentiation. It is also essential for producing interferon-gamma (IFG-g), and mediating responses to viral/bacterial pathogens. Upon cytokine signaling, cytoplasmic STAT4 is phosphorylated, dimerizes, and translocates to the nucleus to bind specific DNA regions. It is inhibited by PIASx (protein inhibitor of activated STAT), which acts as a co-repressor. It is expressed primarily in the thymus, spleen, and peripheral blood leukocytes. Variations in the STAT4 gene are strongly associated with increased risk for autoimmune diseases, including Systemic Lupus Erythematosus (SLE), Rheumatoid Arthritis (RA), Sjögren’s syndrome, and type 1 diabetes. This description may include information annotated by UniProt and/or Google AI.