Product Name:
STAT2-pY690
Product Number:
ab-pn668
Target Full Name: Signal transducer and activator of transcription 2
Target Alias: p113
Product Type Specific: STAT2 phosphosite-specific antibody
Antibody Code: PN668
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y690
Protein UniProt: P52630
Protein SigNET: STAT2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STAT2
Antibody Immunogen Sequence: ERRK(pY)LKHR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E686 to R694. Y690 phosphorylation stimulates transcriptional activity. This has not been identified as an in vivo phosphorylation of STAT2 from high throughput mass spectrometry studies recorded in PhosphoSitePlus. STAT2 is known to be phosphorylated at this site in vitro by JAK1, Lck, and Tyk2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Mouse.
Related Product 1: STAT1 Y701 phosphorylation antibody (Cat. No.: AB-PN666)
Related Product 2: STAT1 S727 phosphorylation antibody (Cat. No.: AB-PN667)
Related Product 3: STAT3 Y705+T708 phosphorylation antibody (Cat. No.: AB-PN539)
Related Product 4: STAT3 S727 phosphorylation antibody (Cat. No.: AB-PN669)
Related Product 5: STAT4 Y693 phosphorylation antibody (Cat. No.: AB-PN670)
Related Product 6: STAT5A Y694 phosphorylation antibody (Cat. No.: AB-PN671)
Related Product 7: STAT5A S780 phosphorylation antibody (Cat. No.: AB-PN672)
Related Product 8: STAT6 Y641 phosphorylation antibody (Cat. No.: AB-PN673)
Scientific Background: STAT2 (Signal transducer and activator of transcription 2) is a transcription factor of the STAT family that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, JAK kinases (TYK2 and JAK1) are activated and catalyze tyrosine phosphorylation of cytoplasmic STAT1 and STAT2. Unlike other STATs, STAT2 generally does not form functional homodimers. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, which enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. It acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at Ser-616 and Ser-637 which activate and inactivate the GTPase activity of DNM1L respectively. It is essential for the innate immune response to viruses (e.g., Influenza A, Hepatitis) by facilitating interferon-stimulated gene (ISG) transcription. Mutations can lead to severe susceptibility to infections (Immunodeficiency 44). High expression is associated with poor prognosis in ovarian cancer and influences breast cancer progression. This description may include information annotated by UniProt and/or Google AI.