Product Name:
STAT1-pY701
Product Number:
ab-pn666
Target Full Name: Signal transducer and activator of transcription 1-alpha/beta
Target Alias: ISGF-3; Signal transducer and activator of transcription 1; Signal transducer and activator of transcription 1, 91kDa; STAT91; Transcription factor ISGF-3 components p91/p84
Product Type Specific: STAT1 phosphosite-specific antibody
Antibody Code: PN666
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y701
Protein UniProt: P42224
Protein SigNET: STAT1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STAT1
Antibody Immunogen Sequence: CGTG(pY)IKTE
Antibody Immunogen Description: Corresponds to amino acid residues G698 to E705. Y701 phosphorylation stimulates transcriptional activity and induces interaction with DNA, PIAS1 and STAT2. This is a major site of in vivo phosphorylation of STAT1 (≥65 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). STAT1 is known to be phosphorylated at this site in vitro by FGFR3, JAK1, JAK2, JAK3, NPM-ALK, Src, and Tyk2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Zebra fish | Fruit fly.
Related Product 1: STAT1 S727 phosphorylation antibody (Cat. No.: AB-PN667)
Related Product 2: STAT2 Y690 phosphorylation antibody (Cat. No.: AB-PN668)
Related Product 3: STAT3 Y705+T708 phosphorylation antibody (Cat. No.: AB-PN539)
Related Product 4: STAT3 S727 phosphorylation antibody (Cat. No.: AB-PN669)
Related Product 5: STAT4 Y693 phosphorylation antibody (Cat. No.: AB-PN670)
Related Product 6: STAT5A Y694 phosphorylation antibody (Cat. No.: AB-PN671)
Related Product 7: STAT5A S780 phosphorylation antibody (Cat. No.: AB-PN672)
Related Product 8: STAT6 Y641 phosphorylation antibody (Cat. No.: AB-PN673)
Scientific Background: STAT1 (Signal Transducer and Activator of Transcription 1-alpha/beta) is a critical protein that acts as a transcription factor, mediating cellular responses to interferons (IFN-a/b/g), cytokines (KITLG/SCF), and growth factors (FGF's that stimulate FGFR1, FGFR2, FGFR3 and FGFR4). STAT1 exists as two isoforms: the full-length 91 kDa STAT1-alpha and the shorter, splice variant STAT1-beta. It is essential for innate and adaptive immunity, controlling gene expression for antiviral, antibacterial, and antiproliferative responses. It is activated via phosphorylation at Tyr-701 and by JAK kinases (JAK1, TYK2) in response to signaling molecules. Upon phosphorylation, STAT1 forms homodimers (Gamma-activated factor - GAF) or heterodimers (with ISGF3G/IRF-9 to form ISGF3) that translocate to the nucleus to induce gene transcription. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, STAT1 is phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6. The Thr-449 phosphorylation also induces STAT1 binding to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription. It maintains immune balance by regulating the IL-17 pathway and mediating the effects of Type I, II, and III interferons. It is also involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine. Viruses (e.g., Ebola, Influenza, SARS-CoV) often evolve mechanisms to inhibit STAT1 phosphorylation, nuclear import, or induce its degradation. Loss-of-Function mutations lead to severe susceptibility to viral, bacterial, and mycobacterial infections. Gain-of-Function mutations causes chronic mucocutaneous candidiasis and autoimmune diseases, often due to impaired IL-17 immunity. . This description may include information annotated by UniProt and/or Google AI.