Product Name:
SRF-pS224
Product Number:
ab-pn664
Target Full Name: Serum response factor
Target Alias: C-fos serum response element-binding transcription factor; MCM1; Serum response factor (C-fos serum response element-binding transcription factor) variant
Product Type Specific: SRF phosphosite-specific antibody
Antibody Code: PN664
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S224
Protein UniProt: P11831
Protein SigNET: SRF
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SRF
Antibody Immunogen Sequence: NSPD(pS)PPR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues N220 to R227. S224 phosphorylation increases transcription and induces interactions with MKL1. This is one of the major sites of in vivo phosphorylation of SRF based on ≥46 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Frog.
Scientific Background: SRF (Serum Response Factor) is a ubiquitously expressed, evolutionarily conserved transcription factor of the MADS-box family that binds to CArG-box DNA motifs. It acts as a master regulator of the actin cytoskeleton, muscle-specific gene expression, cell proliferation, and differentiation by integrating RhoA-mediated signaling with co-activators like MRTFs. SRF binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of immediate early genes (e.g., c-fos) and muscular genes. It plays a role in regulating actin dynamics, which is essential for cell migration and shape. SRF is vital for the development and maintenance of cardiac, skeletal, and smooth muscle tissues. It acts as a downstream target for various pathways, including the MAPK pathway (via ternary complex factors) and RhoA signaling. Together with MRTFA transcription coactivator, it controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. It is required for cardiac differentiation and maturation. SRF activity is often regulated by co-factors such as Myocardin (MYOCD) and Myocardin-related transcription factors (MRTFs), which modulate its function in different cell types. Abnormal SRF expression is linked to cardiovascular diseases, smooth muscle dysfunction, and has potential roles in cancer and diabetes. This description may include information annotated by UniProt and/or Google AI.