Product Name:
SPT5-pT791
Product Number:
ab-pn663
Target Full Name: Transcription elongation factor SPT5
Target Alias: DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; FLJ34157; SPT5H; Suppressor of Ty 5; Tat-cotransactivator 1; Tat-CT1
Product Type Specific: SPT5 phosphosite-specific antibody
Antibody Code: PN663
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T791
Protein UniProt: O00267
Protein SigNET: SPT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SPT5
Antibody Immunogen Sequence: CGSQ(pT)PLQD
Antibody Immunogen Description: Corresponds to amino acid residues G785 to D795. The effect of T791 phosphorylation is unclear. This is a major site of in vivo phosphorylation of SPT5 (≥99 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). SPT5 is known to be phosphorylated at this site in vitro by CDK2, CDK9.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Mouse | Chicken | Frog | Zebra fish.
Scientific Background: SPT5 is a highly conserved, essential protein that functions as a universal transcription elongation factor, travelling with RNA polymerase II (Pol II) to regulate transcription through various stages. It is is a component of the DRB sensitivity-inducing factor complex (DSIF complex) that targets Pol II, to regulates mRNA processing and transcription elongation. DSIF stimulates mRNA capping by increasing the mRNA guanylyltransferase activity of RNGTT/CAP1A. It features an N-terminal NGN domain (which binds to Spt4), multiple KOW domains (involved in protein-protein/nucleic acid interactions), and a C-terminal repeat (CTR) domain. In eukaryotes, Spt5 binds with Spt4 to form the DSIF complex (DRB-sensitivity-inducing factor), which is essential for regulating RNA polymerase II. DSIF (Spt4/Spt5) binds to Pol II, causing promoter-proximal pausing. It is then converted into a positive elongation factor by P-TEFb-mediated phosphorylation of the Spt5 CTR, enabling efficient transcription. It stabilizes the RNA polymerase II elongation machinery on chromatin, preventing early termination and aiding in transcription through nucleosome barriers. Spt5 facilitates co-transcriptional processes like mRNA capping, splicing, and 3' end processing. This description may include information annotated by UniProt and/or Google AI.