Product Name:
SOX9-pS181
Product Number:
ab-pn662
Target Full Name: Transcription factor SOX-9; SRY (sex determining region Y)-box 9
Target Alias: CMD1; CMPD1; SRA1; SRY (sex determining region Y)-box 9
Product Type Specific: SOX9 phosphosite-specific antibody
Antibody Code: PN662
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S181
Protein UniProt: P48436
Protein SigNET: SOX9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SOX9
Antibody Immunogen Sequence: RRRK(pS)VKNGC
Antibody Immunogen Description: Corresponds to amino acid residues R175 to G185. S181 phosphorylation stimulates transcriptional activity by increasing SOX9's DNA binding and transcriptional activation, and regulates cytoskeletal reorganization, intracellular location and molecular association, including inducing interaction with KPNB1. This is a minor site of in vivo phosphorylation of SOX9 (≥20reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). SOX9 is known to be phosphorylated at this site in vitro by PKACa (PRKACA), and ROCK1 (ROK-beta).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Mouse | Platypus | Chicken | Frog.
Scientific Background: SOX9 (Transcription factor SOX-9) is a transcription factor that belonging to the SRY-related HMG-box family. It is essential for skeletal development, chondrogenesis, and male sex determination. Features a dimerization (DIM) region (63-13), and two transactivation domains: TAM (224-307) and TAC (394-509). It binds to DNA to regulate gene expression for cartilage formation, stem cell maintenance, and tissue homeostasis. It binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219. It is essential for cartilage formation by regulating chondrocyte differentiation and secreting cartilage-specific matrix components. It serves as a master regulator in testis formation from bipotential gonads. It is critical for development of the heart, hair follicles, and pancreas. SOX9 remains active throughout life in articular cartilage and is also involved in regulating immune cell differentiation. Overexpression is associated with cancer progression (breast, colorectal) and vascular aging, while deficiencies cause severe skeletal defects. Mutations in the SOX9 gene lead to Campomelic Dysplasia. This description may include information annotated by UniProt and/or Google AI.