Product Name:
SIN3A-pS832
Product Number:
ab-pn655
Target Full Name: Paired amphipathic helix protein Sin3a
Target Alias: DKFZP434K2235; KIAA0700; SIN3 A, transcription regulator; SN3A; Transcriptional corepressor Sin3a
Product Type Specific: SIN3A phosphosite-specific antibody
Antibody Code: PN655
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S832
Protein UniProt: Q96ST3
Protein SigNET: SIN3A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SIN3A
Antibody Immunogen Sequence: CGDL(pS)DVEE
Antibody Immunogen Description: Corresponds to amino acid residues G829 to E836. The effect of S832 phosphorylation is unclear. This is a major site of in vivo phosphorylation site of SIN3A based on ≥69 high throughput mass spectrometry reports recorded in PhosphoSitePlus. SIN3A is known to be phosphorylated at this site in vitro by CK2a1 (CSNK2A1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Sea urchin.
Scientific Background: SIN3A (Paired amphipathic helix protein Sin3a) is a conserved, scaffold protein that acts as a master transcriptional corepressor, regulating gene expression, chromatin structure, and embryonic development. As a central component of the SIN3A-HDAC complex, it recruits histone deacetylases (HDAC1/2) to target genes via four paired amphipathic helix (PAH) domains that serve as docking sites for various transcription factors (e.g., Mxd1, Mad-Max) and chromatin modifying enzymes, mediating repression. SIN3A does not bind DNA directly but acts as a scaffold to bring HDACs to specific loci, promoting deacetylation and a closed chromatin structure. It is essential for maintaining genomic integrity in pluripotent embryonic cells and development. It also regulates cell cycle progression and apoptosis. It interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities, and with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. It acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. It is involved in the control of the circadian rhythms. It is required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. It cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. SIN3A is localized to the nucleus and shows ubiquitous expression, with high levels in testis. It is found in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions. It is required for cortical neuron differentiation and callosal axon elongation. Mutations in SIN3A are associated with a syndrome characterized by developmental delay, intellectual disability, and specific facial dysmorphism. Dysregulation of SIN3A is linked to cancer, as it can function as a tumour suppressor, and its expression is reduced in various human tumours. This description may include information annotated by UniProt and/or Google AI.