Product Name:
SH2BP1-pT925
Product Number:
ab-pn654
Target Full Name: RNA polymerase-associated protein CTR9 homolog
Target Alias: SH2 domain-binding protein 1
Product Type Specific: SH2BP1 (CTR9) phosphosite-specific antibody
Antibody Code: PN654
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T925
Protein UniProt: Q6PD62
Protein SigNET: SH2BP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SH2BP1
Antibody Immunogen Sequence: VNDD(pT)DDDLC
Antibody Immunogen Description: Corresponds to amino acid residues V921 to L929. The effect of T925 phosphorylation is unclear. This is a major in vivo site for phosphorylation of SH2BP1 based on ≥126 mass spectrometry reports recorded in PhosphoSitePlus. SH2BP1 is known to be phosphorylated at this site in vitro by CK2a1 (CSNK2A1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Frog | Zebra fish.
Scientific Background: SH2BP1 (RNA polymerase-associated protein; CTR9 homolog) is a 150 kDa component of the PAF1 complex (PAF1C), which plays a central role in transcriptional regulation by RNA polymerase II. It is a large, nuclear protein (approximately 150 kDa) that acts as a scaffold for the assembly of the PAF1 complex and connects transcription to various histone modifications. The N-terminal two-thirds of the protein contains 15–16 tetratricopeptide repeats (TPRs), which mediate protein-protein interactions and self-association. As part of the PAF1C, SH2BP1 is involved in transcriptional elongation, acting in cooperation with other factors to facilitate RNA polymerase II movement along DNA. It is required for specific histone modifications, including the monoubiquitination of histone H2B (H2BK120ub1) and trimethylation of histone H3 Lys-4 (H3K4me3). It is also involved in regulating the distribution of H3K27me3, acting to counterbalance the Polycomb Repressive Complex 2 (PRC2). PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. It promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. SH2BP1 is crucial for maintaining embryonic stem cell pluripotency and is involved in the expression of Hox and Wnt target genes. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. It has been shown to interact with the dopamine transporter (DAT) in the cytoplasmic compartment, aiding its localization to the plasma membrane. It is required for Hox gene transcription. It is needed for the trimethylation of histone H3 Lys-4 (H3K4me3) on genes involved in stem cell pluripotency. This function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. It is involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway. It may regulate DNA-association of STAT3. While it is ubiquitously expressed, SH2BP1 is primarily found in the nucleus, particularly in speckled patterns. While having tumour-suppressive roles (associated with Wilms tumour), it can also act as an oncogene, for example, by stabilizing the estrogen receptor (ERα) in breast cancer or through interactions with MLL-rearranged proteins in leukemia. This description may include information annotated by UniProt and/or Google AI.