Product Name:
SATB1-pS38
Product Number:
ab-pn653
Target Full Name: DNA-binding protein SATB1
Target Alias: SATB homeobox 1; Special AT-rich sequence-binding protein 1
Product Type Specific: SATB1 phosphosite-specific antibody
Antibody Code: PN653
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S38
Protein UniProt: Q01826
Protein SigNET: SATB1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human SATB1
Antibody Immunogen Sequence: EQNG(pS)PLGC
Antibody Immunogen Description: Corresponds to amino acid residues E34 to G41. The effect of S38 phosphorylation is unclear. This is the major site of in vivo phosphorylation of SATB1 based on ≥171 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish.
Scientific Background: SATB1 (Special AT-rich sequence-binding protein 1) is a critical nuclear matrix protein and global chromatin organizer that regulates gene expression by anchoring DNA to the nuclear matrix, specifically at AT-rich sequences. It belongs to the CUT homeobox family. It is a tetrameric protein that forms a platform for recruiting chromatin-remodeling factors, containing specialized domains (CUT1, CUT2, and a Homeodomain) that bind to "base unpairing regions" (BURs) in DNA. It appears to recognize the sugar-phosphate structure of double-stranded DNA. It functions as a context-dependent regulator, acting as both a transcriptional repressor and activator to manage gene expression. It binds to Matrix Attachment Regions (MARs) and organizes chromatin into loop domains, facilitating higher-order structure and allowing it to regulate multiple genes simultaneously, often over long distances. It is a silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 is important for thymocyte development, T-cell maturation, and immune regulation. It controls T-helper 2 (Th2) differentiation, including repression of IL-5, and regulates genes involved in cell differentiation, proliferation, and apoptosis. It is expressed in specific neurons (e.g., in the cortex), it is involved in synaptic plasticity, and dysfunction has been linked to neurodevelopmental disorders. It is predominantly found in thymus. SATB1 delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumour-suppressor genes. It reprograms chromatin organization and the transcription profiles of breast tumours to promote growth and metastasis. This description may include information annotated by UniProt and/or Google AI.