Product Name:
RXRa-pS260
Product Number:
ab-pn652
Target Full Name: Retinoic acid receptor RXR-alpha
Target Alias: NR2B1; Retinoid X receptor alpha; Retinoid X receptor, alpha
Product Type Specific: RXRa phosphosite-specific antibody
Antibody Code: PN652
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S260
Protein UniProt: P19793
Protein SigNET: RXRa
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human RXRa
Antibody Immunogen Sequence: C(βA)NPS(pS)PNDP
Antibody Immunogen Description: Corresponds to amino acid residues N257 to P264. S260 phosphorylation inhibits transcriptional activity, inhibits interaction with DNA, RARB, RXRA and VDR, and regulates transcription, intracellular location, and protein stability. This is a minor site of in vivo phosphorylation of RXRa based on ≥2 mass spectrometry reports recorded in PhosphoSitePlus. RXRa is known to be phosphorylated at this site in vitro by ERK1 (MAPK3), ERK2 (MAPK1), JNK1 (MAPK8), and JNK2 (MAPK9).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus.
Scientific Background: RXRA (Retinoid X Receptor alpha) is a ~55 kDa nuclear receptor and ligand-dependent transcription factor that belongs to the nuclear hormone receptor family, NR2 subfamily. It plays a critical role in regulating gene expression for cell proliferation and differentiation, apoptosis, lipid metabolism, and embryonic development. It binds to 9-cis retinoic acid and acts as a central dimer partner for various nuclear receptors (e.g., PPARs, RARs) to regulate metabolic and developmental pathways. It forms homo- or heterodimers to bind retinoic acid response elements (RAREs) in target gene promoters.The RAR/RXR heterodimers bind to RARE composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, RXR heterodimers bind corepressors. Ligand binding causes corepressor dissociation and coactivator recruitment, activating transcription. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. RXRA is often considered a "permissive" partner, allowing heterodimerization with other receptors like PPARA, LXR, and VDR to modulate metabolic signaling. RXRA is expressed in many tissues, with notable levels in the liver, lungs, kidneys and heart. This description may include information annotated by UniProt and/or Google AI.