Product Name:
RDBP-pS89+pT91
Product Number:
ab-pn650
Target Full Name: Negative elongation factor E
Target Alias: D6S45; Negative elongation factor E;RNA-binding protein RD; NELFE; NELF-E; NLFE; RD; RD protein; RD RNA binding protein; RDP
Product Type Specific: RDBP phosphosite-specific antibody
Antibody Code: PN650
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S89+T91
Protein UniProt: P18615
Protein SigNET: RDBP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human RDBP
Antibody Immunogen Sequence: CGFKR(pS)R(pT)LEGK
Antibody Immunogen Description: Corresponds to amino acid residues G85 to K95. The effects of S89 and T91 phosphorylation are unclear. These are two of the major sites of in vivo phosphorylation of RDBP based on ≥105 and ≥186 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Frog | Zebra fish.
Scientific Background: RDBP (RD RNA-binding protein; NELFE) is a 45.4–45.6 kDa subunit of the five-component Negative Elongation Factor (NELF) complex. It belongs to the RRM NELF-E family. It plays a critical role in controlling gene expression by regulating RNA polymerase II (Pol II) transcript elongation. RDBP/NELFE works with DSIF (DRB sensitivity-inducing factor) to repress transcription by inducing Pol II pausing shortly after transcription begins. The NELF complex is counteracted by the P-TEFb kinase complex. It features a unique, long region of alternating basic and acidic residues, specifically arginine (R) and aspartic acid (D). While it is proposed to be a RNA-binding protein (RBP) and contains an RNA Recognition Motif (RRM) that binds to nascent TAR RNA to help stabilize the NELF complex at pause sites, its direct RNA-binding has been challenged. RDBP is expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. The NELF complex is involved in HIV-1, potentially recruiting factors to the paused polymerase. RDBP levels have been identified as an independent risk factor for early intrahepatic recurrence of Hepatocellular Carcinoma (HCC). It is expressed in the brain, lung, liver, and other tissues. This description may include information annotated by UniProt and/or Google AI.