Product Name:
RARA-pS77
Product Number:
ab-pn648
Target Full Name: Retinoic acid receptor alpha
Target Alias: Nuclear receptor subfamily 1 group B member 1
Product Type Specific: RARA phosphosite-specific antibody
Antibody Code: PN648
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S77
Protein UniProt: P10276
Protein SigNET: RARA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human RARA
Antibody Immunogen Sequence: PSPP(pS)PPP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues P73 to P80. S77 phosphoryation stimulates transcriptional activity, and regulates protein degradation and molecular association, including inducing interaction with DNA, N-CoR1, Pin1, and inhibiting interaction with SRC-3. This is one of the minor sites of in vivo phosphorylation of RARA based on ≥2 mass spectrometry reports recorded in PhosphoSitePlus. RARA is known to be phosphorylated at this site in vitro by CDK1 (CDC2), and CDK7.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Mouse | Platypus | Chicken | Frog.
Scientific Background: RARA (Retinoic acid receptor alpha) is a nuclear transcription factor that belongs to the nuclear hormone receptor family, NR1 subfamily. It is important for myeloid differentiation (white blood cell maturation), spermatogenesis and skeletal development. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. Formation of a complex of RARA with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression. Together with RXRA, it positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells. In association with HDAC3, HDAC5 and HDAC7 corepressors, plays a role in the repression of microRNA-10a and thereby promotes the inflammatory response. Its deregulation, particularly via the PML-RARA fusion, is the primary cause of acute promyelocytic leukemia (APL). In >98% of APL cases, a chromosomal translocation forms a PML-RARA fusion protein. This chimeric protein fails to respond to normal differentiation signals, blocking white blood cells at the immature promyelocyte stage and promoting leukemia. This description may include information annotated by UniProt and/or Google AI.