Product Name:
PU.1-pS146
Product Number:
ab-pn647
Target Full Name: Transcription factor PU.1
Target Alias: 31 kDa transforming protein; 31 kDa-transforming protein; Hematopoietic transcription factor PU.1; OF; SFPI1; SPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; Spleen focus forming virus (SFFV) proviral integration oncogene spi1
Product Type Specific: PU.1 phosphosite-specific antibody
Antibody Code: PN647
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S146
Protein UniProt: P17947
Protein SigNET: PU.1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PU.1
Antibody Immunogen Sequence: PLEV(pS)DGEAC
Antibody Immunogen Description: Corresponds to amino acid residues P142 to A150. S146 phosphorylation induces transcriptional activation and interaction with DNA, IRF2, IRF4 , IRF8, PIP and PU1. This is one of the major sites of in vivo phosphorylation of PU.1 based on ≥4 mass spectrometry reports recorded in PhosphoSitePlus. PU.1 is known to be phosphorylated at this site in vitro by CK2a1 (CSNK2A1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus | Frog | Zebra fish.
Scientific Background: PU.1 (Transcription factor PU.1) is a crucial Ets-family transcription factor encoded by the SPI1 gene, which belongs to the ETS family. It functions as a master regulator of hematopoietic stem cell differentiation, specifically driving myeloid (monocytes, granulocytes) and B-lymphoid lineage commitment. It forms complex partnerships with other transcription factors (e.g., IRF4, IRF8) to regulate over 3,000 target genes related to immunity, cytokines, and cellular communication. It instructs multipotent progenitor cells to become myeloblasts by initiating myeloid-specific gene programs and suppressing alternative fates (e.g., inhibiting GATA-1). It acts as a pioneer factor, binding the purine-rich "PU-box" (5′-GAGGAA-3′) in enhancers/promoters to activate gene expression and remodel chromatin. It also binds RNA and may modulate pre-mRNA splicing. Overexpression of SPI1 is linked to Friend erythroleukemia, where it prevents terminal differentiation. Mutations or dysregulation of SPI1 are associated with acute myeloid leukemia (AML) and other hematological malignancies. This description may include information annotated by UniProt and/or Google AI.