Product Name:
PPARg-1-pS112
Product Number:
ab-pn644
Target Full Name: Peroxisome proliferator-activated receptor gamma
Target Alias: Nuclear receptor subfamily 1 group C member 3
Product Type Specific: PPARg-1 phosphosite-specific antibody
Antibody Code: PN644
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S112
Protein UniProt: P37231
Protein SigNET: PPARg-1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPARg-1
Antibody Immunogen Sequence: C(βA)EPA(pS)PPYY
Antibody Immunogen Description: Corresponds to amino acid residues E109 to Y116. S112 phosphorylation inhibits transcriptional activity by inhibiting both ligand-independent and ligand-dependent transactivation functions of peroxisome proliferator-activated receptor gamma and targets PPARG to the ubiquitin-proteasome system to regulate cell growth and differentiation. This is a major site of in vivo phosphorylation of PPAR-gamma (≥7 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PPARg-1 is known to be phosphorylated at this site in vitro by CDK5, CDK9, ERK1 (MAPK3), ERK2 (MAPK1), and JNK1 (MAPK8).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Scientific Background: PPARg-1 (Peroxisome proliferator-activated receptor gamma) is a nuclear receptor that belongs to the nuclear hormone receptor family, NR1 subfamily. It binds peroxisome proliferators such as hypolipidemic drugs, prostaglandins and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. It serves as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. It functions in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels. Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are likely modulated through PPARg-1. It also promotes anti-inflammatory M2 macrophage activation and regulates cell cycle arrest. The highest expression of PPARg-1 is in adipose tissue, with lower levels in skeletal muscle, spleen, heart and liver. PPARG is involved in the pathology of diabetes, obesity, cancer, and atherosclerosis. This description may include information annotated by UniProt and/or Google AI.