Product Name:
p53-pT18+pS20
Product Number:
ab-pn638
Target Full Name: Tumour suppressor protein p53 (antigenNY-CO-13)
Target Alias: P53; LFS1; TRP53; FLJ92943; TP53; p53 Tumour Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumour protein p53
Product Type Specific: TP53 transcription protein phosphosite-specific antibody
Antibody Code: PN638
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T18+S20
Protein UniProt: P04637
Protein SigNET: p53
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human p53
Antibody Immunogen Sequence: LSQE(pT)F(pS)DLW(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues L14 to W23. T18 and S20 phosphorylation stimulates binding of CBP/p300, and inhibits binding to its negative regulatory partner MDM2. T18 phosphorylation has not been reported by mass spectrometry studies, and phosphorylation of the S20 site has only been reported once by mass spectrometry, although ≥117 reports for S20 phosphorylation has been made based on Western blotting data. TP53 is known to be phosphorylated in vitro at T18 by AMPKA1, Chk1 (CHEK1), Chk2 (CHEK2), CK1a1 (CSNK1A1), CK1d1 (CSNK1D), DAPK1, DNAPK (PRKDC), VRK1, and VRK2, and at S20 by AMPKa1 (PRKAA1), ATM, CDK5, Chk1 (CHEK1), Chk2 (CHEK2), CK1a1 (CSNK1A1), CK1d1 (CSNK1D), DAPK1, DAPK3 (ZIPK), DNAPK (PRKDC), DRAK1 (STK17A), JNK1 (MAPK8), JNK2 (MAPK9), MAPKAPK2, and Plk3 (CNK). .
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Frog.
Related Product 1: p53 expression antibody (Cat. No.: AB-NN082-2)
Related Product 2: p53 expression antibody (Cat. No.: AB-NN082-3)
Related Product 3: p53 S6+S9 phosphorylation antibody (Cat. No.: AB-PN637)
Related Product 4: p53 S392 phosphorylation antibody (Cat. No.: AB-PN640)
Related Product 5: p53 S15 phosphorylation antibody (Cat. No.: AB-PN859)
Scientific Background: The p53 protein ( tumour protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumour suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5). This description may include information annotated by UniProt and/or Google AI.