Product Name:
Nur77-pS351
Product Number:
ab-pn636
Target Full Name: Nuclear receptor subfamily 4 group A member 1
Target Alias: Early response protein NAK1; GFRP; GFRP1; HMR; N10; N10 nuclear protein; NAK1; NAK-1; NGFIB; NGFI-B; NR4A1; Nuclear hormone receptor NUR/77; Nuclear receptor subfamily 4, group A, member 1; NUR77; Orphan nuclear receptor HMR; TR3; TR3 orphan receptor
Product Type Specific: Nur77 phosphosite-specific antibody
Antibody Code: PN636
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S351
Protein UniProt: P22736
Protein SigNET: Nur77
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Nur77
Antibody Immunogen Sequence: CGRLP(pS)KPKQ
Antibody Immunogen Description: Corresponds to amino acid residues G347 to Q355. S351 phosphorylation inhibits transcriptional activity and regulates apoptosis and cytoskeletal reorganization. This is the major in vivo phosphorylation site in Nur77 (≥4 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Nur77 is known to be phosphorylated at this site in vitro by Akt1 (PKBa), MSK1 (RPS6KA5), PKACa (PRKACA), RSK1 (RPS6KA1), and RSK2 (RPS6KA3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly.
Scientific Background: Nur77 (Nuclear receptor subfamily 4immunitygroup A member 1) is a growth factor-inducible, immediate-early gene protein belonging to the steroid/thyroid hormone receptor superfamily, NR4A subfamily. It features an N-terminal domain, a DNA-binding domain, and a ligand-binding domain. Although classified as an orphan nuclear receptor/transcription factor (lacking a known endogenous ligand), its activity can be modulated by small molecules. By binding to specific DNA sequences in the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' in the promoter regions of target genes, it regulates genes involved in cell survival, proliferation, apoptosis (cell death), and inflammation. It may act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Nur77 can translocation to the mitochondria, where it binds to Bcl-2, converting it from an anti-apoptotic to a pro-apoptotic protein, thus inducing apoptosis. It functions as a negative regulator of inflammation, suppressing NF-κB signaling, which is crucial in macrophage function and immune responses. It promotes gluconeogenesis in the liver, aiding in glucose homeostasis. Its expression is rapidly induced by various stimuli like growth factors and stress. Its activity is also regulated by phosphorylation, such as via the MAPK pathway. It is highly expressed in the brain, indicating roles in cognitive and motor functions, and also found in fetal muscle and adult liver and thyroid. It is involved in regulating cancer cell growth and survival. This description may include information annotated by UniProt and/or Google AI.