Product Name:
NOLC1-pT607+pT610
Product Number:
ab-pn635
Target Full Name: Nucleolar and coiled-body phosphoprotein 1
Target Alias: 140 kDa nucleolar phosphoprotein; HCV NS5A trans-regulated protein 13; KIAA0035; NOPP130; Nopp140; Nucleolar 130 kDa protein; Nucleolar and coiled-body phosphoprotein 1; Nucleolar phosphoprotein p130; P130
Product Type Specific: NOLC1 phosphosite-specific antibody
Antibody Code: PN635
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T607+T610
Protein UniProt: Q14978
Protein SigNET: NOLC1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NOLC1
Antibody Immunogen Sequence: KLQ(pT)PN(pT)FPK
Antibody Immunogen Description: Corresponds to amino acid residues K604 to K613. The effects of T607 and T610 phosphorylation are unclear. These are two of the major in vivo phosphorylation sites in NOLC1 (≥102 and ≥101 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). NOLC1 is known to be phosphorylated in vitro at T607 and T610 by CDK1 (CDC2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Zebra fish.
Scientific Background: NOLC1 (Nucleolar and Coiled-Body Phosphoprotein 1) is a 140 kDa chaperone of the NOLC1 family.It chaperones proteins between the nucleolus and cytoplasm, and plays a critical role in ribosome biogenesis, rRNA transcription, and neural crest development. It acts as a platform for RNA Polymerase I and is highly phosphorylated. It undergoes rapid, massive phosphorylation and dephosphorylation by CK2 and PKC. It has intrinsic GTPase and ATPase activities. It acts as a chaperone that links RNA Polymerase I with enzymes responsible for ribosomal processing. It regulates RNA polymerase I by interacting with the largest subunit (RPA194) and aids in processing small nucleolar ribonucleoproteins (snoRNPs). It is required for neural crest specification. Following monoubiquitination by the BCR(KBTBD8) complex, it associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. It is involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It is primarily localized in the dense fibrillar component of the nucleolus, shuttling between the nucleolus and cytoplasm. NOLC1 is involved in tumorigenesis, often functioning as an oncogene in cancers like breast (TNBC), gastric, and liver cancer, but may act as a tumour suppressor in certain contexts. This description may include information annotated by UniProt and/or Google AI.