Product Name:
NFKB1-pS903+pS907
Product Number:
ab-pn634
Target Full Name: Nuclear factor NF-kappa-B p105 subunit
Target Alias: DNA-binding factor KBF1; EBP-1; KBF1; NF-kappaB; NF-kappa-B1 p84/NF-kappa-B1 p98; NFKB1; NFkB-p50; Nuclear factor NF-kappa-B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; P105; P50
Product Type Specific: NFKB1 phosphosite-specific antibody
Antibody Code: PN634
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S903+S907
Protein UniProt: P19838
Protein SigNET: NFKB1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NFKB1
Antibody Immunogen Sequence: CSQAH(pS)LPL(pS)PAS
Antibody Immunogen Description: Corresponds to amino acid residues S899 to S910. S903 phosphorylation promotes protein proteolysis. This is one of the major in vivo phosphorylation sites in NFKB1 (≥30 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). NFKB1 is known to be phosphorylated at this site in vitro by GSK3b.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque.
Scientific Background: NFkB1 (Nuclear Factor Kappa B Subunit 1) is a 105 kDa precursor protein (p105), which is proteolytically processed by the 26S proteasome to generate the p50 subunit, a crucial DNA-binding component of the NF-κB transcription factor complex. It plays a key role in immune response, inflammation, and cell survival, usually by forming a p50-p65 heterodimer. This protein contains an N-terminal Rel homology domain (RHD) for DNA binding/dimerization and a C-terminal region with ankyrin repeats. NFKB1/p50 has both repressive and activating roles in NF-κB transcription factor complex that regulates genes. NFkB consists of homo- or heterodimeric complexes formed from the five NFkB proteins in mammals: RelA, RelB, c-REL, NFkB1, and NFkB2. They form a variety of homodimers and heterodimers, each binding at kappa-B sites in the DNA, regulating distinct sets of genes. Different dimer combinations act as transcriptional activators or repressors. p50/RelA and p50/RelB heterodimers are transcriptional activators. p50 homodimers are transcriptional repressor, but can act as a transcriptional activators when associated with BCL3. NFkB1 is essential for mediating responses to cytokines, infections, and stress. While the precursor p105 can be found in the cytoplasm, the processed p50 subunit acts as a transcription factor in the nucleus. NFkB1-p105 binds Cot protein kinase, and inhibits Cot-induced MAPK signaling; active Cot is released by proteasome-dependent degradation of p105. NFKB1 is highly expressed in immune cells like macrophages. Dysregulation of NFKB1 is linked to inflammatory bowel disease, diabetes, asthma, and arthritis. Mutations leading to NFKB1 haploinsufficiency (reduced protein levels) are associated with common variable immunodeficiency (CVID). It is involved in cancer progression (angiogenesis, metastasis) and is a key regulator of immediate-early responses to viral infections. This description may include information annotated by UniProt and/or Google AI.