Product Name:
NFAT5-pT135
Product Number:
ab-pn632
Target Full Name: Nuclear factor of activated T-cells 5
Target Alias: KIAA0827; NF-AT5; NFATL1; NFATZ; nuclear factor of activated T cells 5; nuclear factor of activated T-cells 5, tonicity-responsive; OREBP; T cell transcription factor NFAT5; TonE-binding protein; TonEBP; tonicity-responsive enhancer-binding protein
Product Type Specific: NFAT5 phosphosite-specific antibody
Antibody Code: PN632
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T135
Protein UniProt: O94916
Protein SigNET: NFAT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NFAT5
Antibody Immunogen Sequence: QHPS(pT)PKRH(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q131 to H139. T135 phosphorylation increases nuclear localization and induces transcriptional activity. This is one of the major in vivo phosphorylation sites in NFAT5 (≥14 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). NFAT5 is known to be phosphorylated at this site in vitro by CDK5.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse | Platypus.
Related Product 1: NFAT1 S217+S221 phosphorylation antibody (Cat. No.: AB-PN630)
Related Product 2: NFAT3 S213+S217 phosphorylation antibody (Cat. No.: AB-PN631)
Scientific Background: NFAT5 (Nuclear Factor of Activated T cells 5; tonicity-responsive enhancer-binding protein; TonEBP), is a key transcription factor that protects cells from hypertonic/osmotic stress by regulating gene expression for organic osmolytes. It is a Rel-family protein that functions as a homodimer and is activated by high salt/osmolyte concentrations. NFAT5 is the primary transcription factor for osmotic stress, driving the expression of genes involved in cellular adaptation to hypertonic environments. It contains an N-terminal domain (NTD) that senses osmotic changes and undergoes a disorder-to-order transition to activate the protein. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Unlike other NFAT family members, it does not require cooperation with Fos/Jun. Beyond stress response, NFAT5 regulates immune cell functions (T cells and macrophages), contributing to inflammation, proliferation, and survival. It is a constitutively nuclear protein, meaning it is present in the nucleus even without stimulation, and its activation is regulated by post-translational modifications (phosphorylation) and increased DNA binding in response to tonicity. It positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. NFAT5 is essential for maintaining cellular homeostasis, particularly in tissues frequently exposed to high osmotic stress, such as the renal medulla, but it is also found in other tissues like skeletal muscle, heart, brain, and peripheral blood leukocytes. It has been implicated in promoting pro-Tumorigenic behaviors, such as invasion and migration in cancer cells. This description may include information annotated by UniProt and/or Google AI.