Product Name:
NFAT1-pS217+pS221
Product Number:
ab-pn630
Target Full Name: Nuclear factor of activated T-cells, cytoplasmic 2
Target Alias: NFAC2; NFAT pre-existing subunit; NFAT1; NFATP; NF-ATp; Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1
Product Type Specific: NFAT1 phosphosite-specific antibody
Antibody Code: PN630
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S217+S221
Protein UniProt: Q13469
Protein SigNET: NFAT1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NFAT1
Antibody Immunogen Sequence: C(βA)PRT(pS)PIM(pS)PRT
Antibody Immunogen Description: Corresponds to amino acid residues P214 to T224. The effects of S217 and S221 phosphorylation are unclear. These are two of the major in vivo phosphorylation sites in NFAT1 (≥13 and ≥14 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). NFAT1 is known to be phosphorylated at this site in vitro by S217 and S221 by JNK1 (MAPK8).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken.
Related Product 1: NFAT3 S213+S217 phosphorylation antibody (Cat. No.: AB-PN631)
Related Product 2: NFAT5 T135 phosphorylation antibody (Cat. No.: AB-PN632)
Scientific Background: NFAT1 (Nuclear factor of activated T-cells, cytoplasmic 2) is a key transcription factor belonging to the NFAT family, responsible for regulating gene expression in response to sustained calcium signaling. While primarily recognized for its role in immune system development and activation, it is ubiquitously expressed and involved in diverse biological processes including cell cycle regulation, and apoptosis. NFAT1 contains a conserved DNA-binding domain (RHR) and a Regulatory Region (NHR), which is heavily phosphorylated in the cytoplasm in resting cells. It appears as a ~140 kDa protein (phosphorylated, inactive) and a ~120 kDa protein (dephosphorylated, active). Upon stimulation (e.g., TCR stimulation), intracellular calcium levels rise, activating the phosphatase calcineurin. Calcineurin dephosphorylates NFAT1, causing it to translocate from the cytoplasm to the nucleus. Inside the nucleus, Due to the importance of calcineurin in its dephosphorylation, it is indirectly highly sensitive to immunosuppressive drugs, specifically cyclosporin A and FK506. NFAT1 binds to specific DNA sequences, often acting in cooperation with other partners like AP-1 (Fos/Jun complexes) or GATA, to activate or repress gene transcription. It is important for T cell activation, regulating cytokines such as IL-2, IL-4, and TNF-alpha. It is also involved in T cell anergy (a state of unresponsiveness). It acts as a repressor of cell cycle progression in certain contexts, particularly B cells, by binding to promoters and inhibiting the G1/S transition. NFAT1 can induce apoptosis in certain cell types, such as fibroblasts and lymphomas. NFAT1-deficient mice show increased susceptibility to cancer, implicating a tumour-suppressor role in some environments (e.g., in cartilage cells). Conversely, it promotes invasion in some cancers, such as breast cancer. Dysregulated NFAT1 activity is associated with various lymphomas, sarcomas, and carcinomas. Aberrant NFAT1 function is linked to excessive cytokine production in conditions like rheumatoid arthritis. Deficiency in NFAT1 leads to age-dependent chondrocyte dysfunction and osteoarthritic degradation of articular cartilage. This description may include information annotated by UniProt and/or Google AI.